Differential gene expression profiles in the salivary gland of Orius laevigatus

다국어 초록 (Multilingual Abstract)

To determine differential gene expression profiles in the salivary gland of a predatory flower bug species, Oriuslaevigatus (Hemiptera: Anthocoridae), a subtractive cDNA library was constructed by suppression subtractivehybridization. The major transcripts encoding trypsins [30.4% of the total expressed sequence tags (ESTs)]were eliminated from the library in silico, and the remaining salivary gland-specific genes were investigated. Atotal of 501 ESTs were clustered and assembled into 126 contigs (63 multiple sequences and 63 singletons).
Approximately 58% of themwere matched to insect genes. In total, 29 contigs (163 ESTs) in the library were determinedto be differentially transcribed in the salivary gland. A hemolysin-like protein populated 8.2% (42 ESTs)of the library. Hemolysin is known to destruct cells, including blood cells, by forming pores on the cell membrane,likely facilitating O. laevigatus feeding. Digestive enzymes and antimicrobial proteins were also identified fromthe salivary gland-specific library. Genes related to homeostasis, antioxidation, anticoagulation and neuropeptideor peptide hormone processingwere also found to be transcribed in the O. laevigatus salivary gland. Severalmajorcontigs encoded putative secretory salivary proteins, but their functional assignments were not identified inexisting protein databases. The discovery of salivary gland-specific genes supports further studies on biologicallyactive components in the saliva of O. laevigatus.

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