Spider silk fibroin enhances insulin secretion and reduces blood glucose levels in diabetic mice

다국어 초록 (Multilingual Abstract)

Fibroin silk proteins make up the cocoons of silkworms and spider webs and are rich in glycine and alanine residues.
Recent studies have shown that silk fibroin hydrolysate from silkworms improves blood glucose and lipidmetabolism. In the present study, we investigated the anti-diabetic effects of spider silk fibroin protein in type 2diabetic mice. Recombinant AvMaSp-R, which consists of the 240 amino acid repetitive domain of majorampullate silk protein (AvMaSp) from the spider Araneus ventricosus, was produced in baculovirus-infectedinsect cells. We tested the effects of oral AvMaSp-R administration on serum insulin and blood glucose levelsin diabetic mice and found that AvMaSp-R increases serum insulin levels and reduces blood glucose levels indiabetic mice. Consequently, our results are the first to provide evidence that silk fibroin protein from spidersenhances insulin secretion, which leads to reduced blood glucose levels in type 2 diabetic mice.

다국어 초록 (Multilingual Abstract)

Fibroin silk proteins make up the cocoons of silkworms and spider webs and are rich in glycine and alanine residues.
Recent studies have shown that silk fibroin hydrolysate from silkworms improves blood glucose and lipidmetabolism. In the present study, we investigated the anti-diabetic effects of spider silk fibroin protein in type 2diabetic mice. Recombinant AvMaSp-R, which consists of the 240 amino acid repetitive domain of majorampullate silk protein (AvMaSp) from the spider Araneus ventricosus, was produced in baculovirus-infectedinsect cells. We tested the effects of oral AvMaSp-R administration on serum insulin and blood glucose levelsin diabetic mice and found that AvMaSp-R increases serum insulin levels and reduces blood glucose levels indiabetic mice. Consequently, our results are the first to provide evidence that silk fibroin protein from spidersenhances insulin secretion, which leads to reduced blood glucose levels in type 2 diabetic mice

참고문헌 (Reference)

1 Gannon, M. C., "The metabolic response to ingested glycine" 76 : 1302-1307, 2002

2 Gotoh, K., "Sulfated fibroin, a novel sulfated peptide derived from silk, inhibits human immunodeficiency virus replication in vitro" 64 : 1664-1670, 2000

3 Kluge, J. A., "Spider silks and their applications" 26 : 244-251, 2008

4 Lewis, R. V., "Spider silk: ancient ideas for new biomaterials" 106 : 3762-3774, 2006

5 Hyun, C. K., "Soluble fibroin enhances insulin sensitivity and glucose metabolism in 3 T3-L1 adipocytes" 134 : 3257-3263, 2004

6 Altman, G. H., "Silk-based biomaterials" 24 : 401-416, 2003

7 Sun-Gil Do, "Silk fibroin hydrolysate exerts an anti-diabetic effect by increasing pancreatic β cell mass in C57BL/KsJ-db/db mice" 대한수의학회 13 (13): 339-344, 2012

8 Cong, L., "Reducing blood glucose level in TIDMmice by orally administering the silk glands of transgenic hIGF-I silkworms" 410 : 721-725, 2011

9 Alvarado-Vásquez, N., "Oral glycine administration attenuates diabetic complications in streptozotocin-induced diabetic rats" 79 : 225-232, 2006

10 Lee, K. S., "Molecular cloning and expression of the C-terminus of spider flagelliformsilk protein fromAraneus ventricosus" 32 : 705-712, 2007

11 이광식, "Molecular cloning and characterization of the partial major ampullate silk protein gene from the spider Araneus ventricosus" 한국응용곤충학회 15 (15): 641-646, 2012

12 Zhou, C. Z., "Fine organization of Bombyx mori fibroin heavy chain gene" 28 : 2413-2419, 2000

13 Jung, E. Y., "Feeding silk protein hydrolysates to C57BL/KsJ-db/db mice improves blood glucose and lipid profiles" 30 : 783-790, 2010

14 González-Oritiz, M., "Effect of glycine on insulin secretion and action in healthy first-degree relatives of type 2 diabetes mellitus patients" 33 : 358-360, 2001

15 Alvarado-Vásquez, N., "Effect of glycine in streptozotocin-induced diabetic rats" 134 : 521-527, 2003

16 Schwartz, M. W., "Cooperation between brain and islet in glucose homeostasis and diabetes" 503 : 59-66, 2013

17 Ayoub, N. A., "Blueprint for a high-performance biomaterial: full-length spider dragline silk genes" 2 : e514-, 2007

18 Igarashi, K., "Blood pressure-depressing activity of a peptide derived from silkworm fibroin in spontaneously hypertensive rats" 70 : 517-520, 2006

19 Park, K. J., "Antigenotoxicity of peptides produced from silk fibroin" 38 : 411-418, 2002

20 Anuradha, C. V., "Amino acid support in the prevention of diabetes and diabetic complications" 10 : 8-17, 2009