Helicobact er py lori 의 간균형에서 구균형으로의 형태 변화에 따른 항원 발현의 변화에 관한 연구 = Changes in the Antigenic Profiles during Coccoid Conversion of Helicobacterp ylori

다국어 초록 (Multilingual Abstract)

Background/Aims: There are several opinions whether the coccoid forms of Helicobacter pylori (H.
pylori) are viable, infective or degenerative. The aims of this study were to induce coccoid conver-sion
of H. pylori, to study antigenic evolutions during this conversion, and then, to elucidate the
viability of coccoid forms. Methods: The antigenic evolution during coccoid conversion of H. pylori
was studied by Western blotting using different sera from forty six patients who were H. pylori posi-tive.
These sera were used to obtain the total antigens of the strain cultured for 2 days (100% rod)
and for 15 days (>99% coccoid). Results: The antigenic profiles were not changed in 47.8% (22/46
cases) and were changed in 52.2% (24/46 cases) during coccoid conversion. Antigenic fractions
disappeared during coccoid conversion were belonged to protein bands at 120 kDa (corresponding
to CagA) and at 35 kDa (corresponding to porin or adhesin). Conclusions: The result showed that
coccoid forms of H. pylori retain the same cellular structures as rod form, and some of the antigens
disappeared during coccoid conversion. Therefore, coccoid form might be viable, and represent one
of the stages of biological cycle of H. pylori. (Kor J Gastroenterol 1999;33:20 - 26)