Effects of fresh mineralized dentin and cementum on socket healing: a preliminary study in dogs

다국어 초록 (Multilingual Abstract)

Objectives: Dentin is composed of many minerals and growth factors. Based on this composition, we studied its effect as a possible regenerative material for alveolar healing.
Materials and Methods: This study was conducted using four 2.5-year-old mongrel dogs (male; weight, 25 to 30 kg). The third mandibular premolars were carefully mobilized with a dental elevator and then removed using forceps. The crown portions of the extracted teeth were removed with cutters, and the root portions of the remaining teeth were collectively trimmed as closely as possible to 350 to 500 μm. Dentin and cementum (DC) chips harvested from the extracted teeth were soaked in blood and packed into the fresh sockets (autograft). Biopsies were performed at the ends of day 14 and day 56 following implantation. Data were expressed as mean±standard deviation and compared with t-test results.
Results: The ratio of S A (bone) to total area of each probe was determined and was 170±16 μm 2 for the control group and 71±14 μm 2 for the DC group, a significant difference (P<0.05).
Conclusion: DC particulate grafts offered no improvement in bone regeneration in alveolar extraction sockets.

목차 (Table of Contents)

• I. Introduction
• II. Materials and Methods
• III. Results
• IV. Discussion
• V. Conclusion

참고문헌 (Reference)

1 정혜린, "미니피그에서 자가치아뼈 이식의 골형성 효과에 대한 연구" 대한구강악안면외과학회 37 (37): 375-379, 2011

2 Tan WL, "systematic review of post-extractional alveolar hard and soft tissue dimensional changes in humans" 23 (23): 1-21, 2012

3 Urist MR, "Transmembrane bone morphogenesis across multiple-walled diffusion chambers. New evidence for a diffusible bone morphogenetic property" 112 : 612-619, 1977

4 Huggins C, "Transformation of fibroblasts by allogeneic and xenogeneic transplants of demineralized tooth and bone" 132 : 1250-1258, 1970

5 Vignoletti F, "Surgical protocols for ridge preservation after tooth extraction. A systematic review" 23 (23): 22-38, 2012

6 Dimova C, "Socket preservation procedure after tooth extraction" 587 : 325-330, 2014

7 Irinakis T, "Rationale for socket preservation after extraction of a single-rooted tooth when planning for future implant placement" 72 : 917-922, 2006

8 Finkelman RD, "Quantitation of growth factors IGF-I, SGF/IGF-II, and TGF-beta in human dentin" 5 : 717-723, 1990

9 Bessho K, "Purification of rabbit bone morphogenetic protein derived from bone, dentin, and wound tissue after tooth extraction" 48 : 162-169, 1990

10 Jung RE, "Postextraction tissue management: a soft tissue punch technique" 24 : 545-553, 2004

11 Gomes MF, "Optical density of bone repair after implantation of homogenous demineralized dentin matrix in diabetic rabbits" 22 : 275-280, 2008

12 Reed MG, "One-stop stereology: the estimation of 3D parameters using isotropic rulers" 239 : 54-65, 2010

13 Butler WT, "Noncollagenous proteins of a rat dentin matrix possessing bone morphogenetic activity" 56 : 228-232, 1977

14 Lewandrowski KU, "Improved osteoinduction of cortical bone allografts:a study of the effects of laser perforation and partial demineralization" 15 : 748-756, 1997

15 Murata M, "Human acid-insoluble dentin with BMP2 accelerates bone induction in subcutaneous and intramuscular tissues" 118 : 438-441, 2010

16 Botticelli D, "Hard-tissue alterations following immediate implant placement in extraction sites" 31 : 820-828, 2004

17 Kim YK, "Development of a novel bone grafting material using autogenous teeth" 109 : 496-503, 2010

18 Sattayasanskul W, "Dense hydroxyapatite root replica implantation: measurement of mandibular ridge preservation" 3 : 203-207, 1988

19 Wang X, "Cancellous bone lamellae strongly affect microcrack propagation and apparent mechanical properties: separation of patients with osteoporotic fracture from normal controls using a 2D nonlinear finite element method (biomechanical stereology)" 42 : 1184-1192, 2008

20 Reddi AH, "Bone matrix in the solid state: geometric influence on differentiation of fibroblasts" 15 : 1-18, 1974

21 Bang G, "Bone induction in excavation chambers in matrix of decalcified dentin" 94 : 781-789, 1967

22 Yeomans JD, "Bone induction by decalcified dentine implanted into oral, osseous and muscle tissues" 12 : 999-1008, 1967

23 Murata M, "Bone engineering using human demineralized dentin matrix and recombinant human BMP2" 14 : 80-81, 2005

24 Devecioğlu D, "Biomaterials in periodontal regenerative surgery: effects of cryopreserved bone, commercially available coral, demineralized freeze-dried dentin, and cementum on periodontal ligament fibroblasts and osteoblasts" 19 : 107-120, 2004

25 Kim YK, "Analysis of the inorganic component of autogenous tooth bone graft material" 11 : 7442-7445, 2011

26 Nampo T, "A new method for alveolar bone repair using extracted teeth for the graft material" 81 : 1264-1272, 2010