The cold shock proteins induced by temperature shift from 37℃ to 10℃ were assayed by the two-dimensional polyacrylamide gel electrophoresis after the proteins were pulse-labeled with ^(35)S-methionine. The cold shock proteins were quite stable eve...
The cold shock proteins induced by temperature shift from 37℃ to 10℃ were assayed by the two-dimensional polyacrylamide gel electrophoresis after the proteins were pulse-labeled with ^(35)S-methionine. The cold shock proteins were quite stable even if the temperature was returned to 37℃. The cloned recA gene was used to understand the molecular mechanism of the cold shock response. Synthesis of RecA protein from the recA gene harbored on the multicopy plasmid was increased upon cold shock, suggesting that the cold shock response involves the regulation by unlimited cellular factors such as RNA polymerase.