(The) Effects of Herbal Formula PM014 on a Murine Model of Chronic Obstructive Pulmonary Disease = 만성폐쇄성폐질환 흰쥐모델에 대한 한약조성물 PM014의 효과

다국어 초록 (Multilingual Abstract)

The Effects of Herbal Formula PM014 on a Murine Model of
Chronic Obstructive Pulmonary Disease

Jae-Hun Choi
Graduate School of Biological Sciences of Oriental Medicine
KyungHee University, Seoul, Korea.
Directed by Prof. Min-Kyu Shin, Ph.D. & Prof. Hong-Yeoul Kim, Ph.D.

Objective: This study was conducted to evaluate the protective effects of PM014 on LPS-induced chronic obstructive pulmonary disease (COPD).

Materials and Methods: In this study, Balb/c mice were treated once with PM014 at various concentrations (100, 300, 560mg/kg wt) via an oral injection. Two hours later, lipopolysaccaride (LPS) was administrated to the mice to induce a disease that resembles COPD. The numbers of total cells and neutrophils in the bronchoalveolar lavage fluid were then counted and the levels of the pro-inflammatory cytokines, TNF-α and IL-6, were measured. For histologic analysis, periodic acid Schiff (PAS) and hematoxylin and eosin (H&E) stains were evaluated. Finally, the levels of neutrophil elastease (NE) and proliferating cell nuclear antigen (PCNA) were assessed by immunohistochemistry.

Results: On day 3 and 7 after LPS stimulation, the numbers of neutrophils and total cells were significantly lower in the PM014 group than the COPD group. In addition, the TNF-α and IL-6 levels were decreased in the PM014 group. Furthermore, histological analysis demonstrated that PM014 attenuated the effects of LPS-induced lung inflammations.

Conclusions: These data suggest that PM014 exerts protective effects against LPS-induced lung inflammation. Therefore, this herbal medicine may represent a novel therapeutic agent for lung inflammation in general, as well as a specific agent for the treatment of COPD.

국문 초록 (Abstract)

만성폐쇄성폐질환 흰쥐모델에 대한 한약조성물 PM014의 효과

최재훈
경희대학교 대학원 한의생명과학과
지도교수 신민규 & 김홍렬

본 연구는 Lipopolysaccaride (LPS) 로 유도된 만성폐쇄성폐질환 (COPD) 에 대한 한약조성물 PM014의 염증억제효과를 알아보기 위한 실험이다.
한약조성물 PM014를 여러 가지 농도 (100, 300, 560mg/kg wt) 로 Balb/c 흰쥐에게 경구투여하고 2시간 후에 LPS를 코로 투여하여 만성폐쇄성폐질환를 유도하였고, 이후 3일째와 7일째에 총세포수와 호중구수, 염증전구물질인 TNF-α와 IL-6를 측정하였다. 조직학적 분석을 위해 periodic acid Schiff (PAS) 와 hematoxylin and eosin (H&E) 염색을 하여 평가하였다. Immunohistochemistry (IHC) 을 이용하여 Neutrophil elastease (NE) 와 Proliferating cell nuclear antigen (PCNA) 를 평가해보았다.
그 결과, LPS 투여 후 3일과 7일 후 COPD군에 비하여 PM014군 에서 호중구수와 총세포수가 현저하게 감소된 것으로 나타났다. TNF-α 및 IL-6 수치도 PM014군에서 현저하게 감소되었다. 또한 조직학적 실험결과들도 LPS로 유도된 폐의 염증을 감소시킴을 보여주었다.
따라서 한약조성물 PM014는 LPS로 유도된 만성폐쇄성폐질환에 대한 염증억제효과가 있음을 알 수 있다. 이는 만성폐쇄성폐질환에 대한 새로운 치료제로서 한약의 활용가능성을 보여준다고 하겠다.

목차 (Table of Contents)

• I. Introduction 1
• II. Materials and methods 3
• 1. Materials 3
• 2. Animals 4
• 3. Induction of COPD and treatment of animals 4
• 4. Analysis of BAL cells 5
• 5. Measurements of IL-6 and TNF-α in bronchoalveloar lavage fluid using ELISA 5
• 6. Preparation of lung tissues, histology, and IHC 6
• 7. Statistical analysis 8
• III. Results 9
• 1. The effects of PM014 on immune cell profiles in BAL fluid 9
• 2. The effects of PM014 on IL-6 and TNF-α in BAL fluid 12
• 3. The effects of PM014 on lung inflammation 15
• 4. The effects of PM014 on goblet cells 19
• 5. Immunohistochemical findings of PM014 19
• IV. Discussion 23
• V. Conclusion 27
• VI. References 28