Preparation and Characterization of Acid and Pepsin-soluble Collagens from Scales of Croceine and Redlip Croakers

다국어 초록 (Multilingual Abstract)

Acid-soluble (ASC) and pepsin-soluble collagen (PSC) from scales of croceine croaker (ASCPC and PSC-PC) and redlip croaker (ASC-PP and PSC-PP) were prepared with yields of 0.37±0.08% (ASCPC), 1.09±0.23% (PSC-PC), 0.42±0.09% (ASC-PP), and 1.14±0.30% (PSC-PP) (dry scale basis). Gly (347.1- 359.8 residues/1,000 residues) was the major amino acid. Contents of imino acid were between 189.4 and 192.9 residues/1,000 residues in all 4 collagens. ASC-PC and ASC-PP were type I collagens. Differences in subunit components between ASC and PSC were observed. Denaturation temperature values of ASC-PC, ASC-PP, PSC-PC, and PSC-PP were 30.7, 30.1, 27.5, and 26.6℃, respectively. All 4 collagens were soluble at pH 1-4. Values declined when NaCl concentrations exceeded 2%. Freeze-dried collagens showed loose, fibrous, and porous structures. The 4 scale collagens can be alternatives to collagen from terrestrial animals for applications in functional food, cosmetic, and biomedical industries.

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