인간 피부 상재균과 병원성 세균에 대한 Paenibacillus elgii DS381과 그 항균물질의 항균활성 = Antimicrobial activity by Paenibacillus elgii DS381 and its antimicrobial substances against microbial residents on human skin and pathogenic bacteria

국문 초록 (Abstract)

이 연구는 분리된 토양 세균에 의해 생성된 항균물질의 효과를 평가하기 위해 수행되었다. 2000여개의 세균 분리주 중 Paenibacillus elgii DS381이 여러 인간 피부 상재균과 병원성 세균에 대해 높은 항균활성을 나타내었다. DS381 균주는 agar well diffusion test에서 모든 대상 세균과 효모에 대해 15.3~26.0 mm 직경의 저해대를 형성하였다. DS381이 생성한 항균 펩티드는 모든 대상 미생물에 낮은 최소저해농도 (0.039-5.000 mg/ml)를 나타내었다. DS381 균주는 lipopeptide 같은 생물계면활성제 생산을 나타내었는데, 배양 상등액의 표면장력을 60.0에서 40.3 mN/m으로 낮추었다. DS381은 또한 $1.56{\pm}0.13U/ml$의 chitinase 활성도 나타내었다. 이 결과들은 P. elgii DS381이 일부 중요한 인간 피부 상재균과 병원성 세균에 대한 효율적인 생물제어제로 사용될 수 있음을 가리킨다.

다국어 초록 (Multilingual Abstract)

This study was carried out to evaluate effects of antimicrobial substances produced by isolated soil bacteria. Among two thousands of bacterial isolates Paenibacillus elgii DS381 exhibited high antimicrobial activities against several microbial residents on human skin and pathogenic bacteria. DS381 showed 15.3~26.0 mm inhibition zone diameter against all target bacteria and yeast in agar well diffusion test. Antimicrobial peptide produced by DS381 indicated low minimum inhibitory concentration (0.039-5.000 mg/ml). DS381 produced biosurfactant such as lipopeptide, and surface tension of culture supernatant of DS381 reduced from 60.0 to 40.3 mN/m. DS381 also showed $1.56{\pm}0.13U/ml$ of chitinase activity. These results suggest that Paenibacillus elgii DS381 may be utilized as an efficient biocontrol agent against some important human skin microbes and pathogenic bacteria.

참고문헌 (Reference)

1 구정은, "화장품에 사용되는 천연방부제 연구 동향" 한국피부과학연구원 11 (11): 835-844, 2013

2 김태완, "해양유래미생물 항균 활성 Bacillus sp. 2-4 균주의 대량 배양방법" 한국수산해양교육학회 30 (30): 123-131, 2018

3 강범룡, "생물적 방제균 Bacillus amyloliquefaciens LM11의 유래생물계면활성물질과 항균활성과의 상관관계" 한국식물병리학회 23 (23): 177-185, 2017

4 J. Behravan, "Survey of bacteriological contamination of cosmetic creams in Iran (2000)" Wiley 44 (44): 482-485, 2005

5 Basma Ghorbel, "Stability studies of protease from Bacillus cereus BG1" Elsevier BV 32 (32): 513-518, 2003

6 Sharma D., "Simultaneous production of biosurfactants and bacteriocins by probiotic Lactobacillus casei MRTL3" 2014 : 698713-, 2014

7 M. D. Lundov, "Recalls of microbiologically contaminated cosmetics in EU from 2005 to May 2008" Wiley 30 (30): 471-474, 2008

8 STEPHANIE WONG, "Recalls of Foods and Cosmetics Due to Microbial Contamination Reported to the U.S. Food and Drug Administration" International Association for Food Protection 63 (63): 1113-1116, 2000

9 Anand Nagpure, "Purification and characterization of an extracellular chitinase from antagonistic Streptomyces violaceusniger" Wiley 53 (53): 429-439, 2013

10 Marco JLD, "Production of hydrolytic enzyme by Trichoderma isolates with antagonistic activity against Crinipellis perniciosa, the causal agent of witches broom of cocoa" 34 : 33-38, 2003

11 Parameswaran Binod, "Production and purification of extracellular chitinases from Penicillium aculeatum NRRL 2129 under solid-state fermentation" Elsevier BV 36 (36): 880-887, 2005

12 Lígia R. Rodrigues, "Physicochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53" Elsevier BV 49 (49): 79-86, 2006

13 Issam AL-Ani, "Pharmacological synergism of bee venom and melittin with antibiotics and plant secondary metabolites against multi-drug resistant microbial pathogens" Elsevier BV 22 (22): 245-255, 2015

14 Stephen A. Cochrane, "Lipopeptides from Bacillus and Paenibacillus spp.: A Gold Mine of Antibiotic Candidates" Wiley 36 (36): 4-31, 2016

15 Murugan G., "Isolation, screening and production of biosurfactant by Pseudomonas sp. isolated from mangrove forest soil using coconut oil cake as substrate" 5 : 251-257, 2011

16 Guo Y, "Isolation of a Paenibacillus sp. strain and structural elucidation of its broad-spectrum lipopeptide antibiotic" 24 : 3156-3165, 2012

17 Xue-Chang Wu, "Isolation and partial characterization of antibiotics produced by Paenibacillus elgii B69" Oxford University Press (OUP) 310 (310): 32-38, 2010

18 Bassam Alkotaini, "Isolation and identification of a new intracellular antimicrobial peptide produced by Paenibacillus alvei AN5" Springer Nature 30 (30): 1377-1385, 2014

19 Naing KW, "Isolation and characterization of an antimicrobial lipopeptide produced by Paenibacillus ehimensis MA2012" 55 : 857-868, 2014

20 Qiao N., "Isolation and characterization of a novel biosurfactant produced by hydrocarbon-degrading bacterium Alcanivorax dieselolei B-5" 108 : 1207-1216, 2013

21 Hanen Ben Ayed, "Isolation and biochemical characterisation of a bacteriocin-like substance produced by Bacillus amyloliquefaciens An6" Elsevier BV 3 (3): 255-261, 2015

22 M. Nagarajkumar, "Involvement of secondary metabolites and extracellular lytic enzymes produced by Pseudomonas fluorescens in inhibition of Rhizoctonia solani, the rice sheath blight pathogen" Elsevier BV 159 (159): 73-81, 2004

23 S.W Budi, "Hydrolytic enzyme activity of Paenibacillus sp. strain B2 and effects of the antagonistic bacterium on cell integrity of two soil-borne pathogenic fungi" Elsevier BV 15 (15): 191-199, 2000

24 Zou YY, "Enhanced antimicrobial activity of nisin in combination with allyl isothiocyanate against Listeria monocytogenes, Staphylococcus aureus, Salmonella typhimurium and Shigella boydii" 48 : 324-333, 2012

25 White IR., "Contact Dermatitis" Springer 493-506, 2006

26 Noha H. Youssef, "Comparison of methods to detect biosurfactant production by diverse microorganisms" Elsevier BV 56 (56): 339-347, 2004

27 Lim JH, "Characterization of the bacterial cell wall lytic enzyme produced by Aspergillus sp. HCLF-4" 37 : 15-20, 2001

28 Jie Jiang, "Characterization of a novel bacteriocin produced by Lactobacillus sakei LSJ618 isolated from traditional Chinese fermented radish" Elsevier BV 23 (23): 338-344, 2012

29 Ansari A, "Bacteriocin (BAC-IB17): Screening, isolation and production from Bacillus subtilis KIBGE IB-17" 25 : 195-201, 2012

30 Lou Z, "Antibacterial activity and mechanism of action of chlorogenic acid" 76 : 398-403, 2011

31 Lee JH, "A study of microbiological contamination after use of lip makeup tester" Sookmyung Women's University 2017