Thermus sp. Sa-15에 의한 耐熱性 Lactate Dehydrogenase의 特性 = Characteristics of Thermostable Lactate Dehydrogenase by Thermus sp. Sa-15

국문 초록 (Abstract)

두엄등 비교적 자연조건이 고온으로 유지되는 곳에서 균원시료를 채취하여 열안정성의 lactate dehydrogenase를 생성하는 한 균주를 분리하였다.
선정된 균주는 고온생육의 호기성이며 gram 음성의 무포자 장간균이고 비운동성인 Thermus속이었다. 처리한 항생물질에 모두 감수성이고, 균의 생육온도는 45˚∼75℃이며 최적 생육온도는 60℃이고, lactate dehydrogenase 활성도는 생균체 g당 15.6unit이었다. 그리고 본 부분정제효소의 최적 pH는 8.0이며 최적 활성온도는 정반응이 70℃이었다. 또한 60℃에서 130분동안 처리시 안정하고 80℃에서는 20분동안 50%가 실활하였다.

다국어 초록 (Multilingual Abstract)

A bacterium which produced thermostable lactate dehydrgenase(EC 1. 1. 1. 27) was isolated from the compost. Microbiological characteristics of Thermus sp. strain was aerobic, non-motile, Gram-negative and 0.8-1.0×7-10㎛ in size and was sensitive to several antibiotics tested. Temprature range for growth of the strain was 45∼75℃, optimum was 60℃.
Optimum pH for partial purified enzyme reaction was 8.0 and optimum temperature was 70℃ at pyruvate reduction.
For pyruvate reduction, the enzyme was stable for 130 min. at 60℃, and its residual activity was 50% for 20 min. at 80℃.