pCrc44-rbcL-AAD, consist of rbcL promotor and aminoglycoside adenyl transferase (aadA) structural gene in atpB region of Chlamydomonas reinhardtii chloroplast DNA, was transformed to Chlamydomonas reinhardtii chloroplast by microprojectile bombardment...
pCrc44-rbcL-AAD, consist of rbcL promotor and aminoglycoside adenyl transferase (aadA) structural gene in atpB region of Chlamydomonas reinhardtii chloroplast DNA, was transformed to Chlamydomonas reinhardtii chloroplast by microprojectile bombardment.
37 clonies were selected as photosynthesis competent cell line on HS minimal medium, among them, 12 clonies showed spectinomycin resistant as a result of expression of aadA. All transformed cell line showed two distinctivly hybridized bands,4.1kb and 3.1kb, by using 0.5kb atpB fragment for probe. It meant that all of them were heterokaryon of normal and aadA transformed cells.