Overexpression of the intertidal seagrass 14-3-3 gene ZjGRF1 enhances the tolerance of transgenic Arabidopsis to salt and osmotic stress

다국어 초록 (Multilingual Abstract)

Salinity afects the physiological activities of seagrass by changing the osmotic pressure of seagrass cells, which then afects the structure and function of seagrass community. In this study, we created transgenic Arabidopsis that overexpressed the ZjGRF1 gene from the intertidal seagrass Zostera japonica. We found that transgenic ZjGRF1 Arabidopsis has stronger tolerance to salt stress and osmotic stress than wild type. Under salt stress, the content of reactive oxygen species in transgenic ZjGRF1 Arabidopsis was lower than that in wild-type Arabidopsis. The viability of root cells in transgenic ZjGRF1 Arabidopsis was higher than that of wild type under salt stress. Under salt stress, transgenic ZjGRF1 Arabidopsis accumulated more proline and had higher antioxidant enzyme activities. The rate of water loss rate from overexpressed plants was lower than that of the wild-type plants. The resistance of overexpressed plants to osmotic stress may be enhanced by the ABA-dependent pathway. Our results confrm that ZjGRF1 enhances tolerance to salt and osmotic stress in Z. japonica.

참고문헌 (Reference)

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