滋陰降火湯(자음강화탕)이 Propylthiouracil, PTU로 유발된 Rat의갑상선기능저하증에 미치는 영향 = Effects of Jaeumkanghwa-tang on the Rat Hypothyroidism Induced by Propylthiouracil, PTU

다국어 초록 (Multilingual Abstract)

This study was to evaluate the effect of Jaeumkanghwa-tang (JEKHT)on the propylthiouracil (PTU)-induced rat hypothyroidism. Methods: Six groups, each of 8 rats per group were used in the present study- intact vehicle control, PTU control, Levothyroxine (LT4), JEKHT 500, 250 and125 mg/kg treated groups. JEKHT were administered once a day for 42 days asan oral dose of 500, 250 and 125 mg/kg, and hypothyroidism was induced by dailysubcutaneous treatment of PTU 10 mg/kg for 28 days. The changes on the bodyand organ weight, serum hormone and lipid profiles, liver and testis antioxidantdefense factors were observed with histopathology of organs. Results were comparedwith LT4 0.5 mg/kg intraperitoneally treated rats in this experiment. Results: PTU treatment, marked decrease of body weight, increases of thyroidweight, decreases of liver, testis, epididymis and prostate weights, decreases ofserum Tri-iodothyronine (T3), and Thyroxine (T4) level with increase of serumThyroid-stimulating hormone (TSH) level, decreases of serum testosterone anddihydrotestosterone (DHT) level with increases of serum Follicular stimulatinghormone (FSH) level, increases of serum High density lipoprotein (HDL), decreaseof triglyceride content, increase of serum Aspartate aminotransferase (AST) level, decreases of liver and testis antioxidant defense factors were observed. In addition, marked hyperplasia of follicular cells with decreases of follicular colloid contentsand diameters was additionally demonstrated with the decrease of hepatocyte numbersper unit area due to hypertrophy of hepatocytes related to lipid droplet depositions, increase of a/oligospermatic epididymal tubules with epididymal atrophic changes, seminiferous tubular atrophy with decrease of stage I~Ⅱ seminiferous tubules intestis, prostate tubular atrophic changes at histopathological inspections. However, these PTU induced hypothyroidism and related hepatic and male reproductive organdamages were favorably and dose-dependently inhibited by treatment of JEKHT500, 250 and 125 mg/kg, and JEKHT also effectively regulated the PTU-inducedabnormal antioxidant defense factor changes in the both liver and testis. Conclusions: JEKHT 500, 250 and 125 mg/kg dose-dependently inhibited PTUinducedhypothyroidism and related liver and male reproductive organ damages in rats.