Effects of method and duration of restraint on stress hormones and meat quality in broiler chickens with different body weights

다국어 초록 (Multilingual Abstract)

Objective: The study was designed to investigate the effects of restraint method, restraint duration, and body weight on stress-linked hormones (corticosterone, adrenaline, and noradrenaline), blood biochemical (namely glucose and lactate), and the meat quality in broiler chickens.
Methods: A total of 120 male broiler chickens (Cobb 500) were assigned to a 2×3×2 factorial arrangement in a completely randomized design using two restraint methods (shackle and cone), three durations of restraint (10, 30, and 60 s), and two categories of live body weight (1.8±0.1 kg as lightweight and 2.8±0.1 kg as heavyweight).
Results: Irrespective of the duration of restraint and body weight, the coned chickens were found to have lower plasma corticosterone (p<0.01), lactate (p<0.001), lower meat drip loss (p<0.01), cooking loss (p<0.05), and higher blood loss (p<0.05) compared with their shackled counterparts. The duration of restraint had significant effects on the meat initial pH (p<0.05), ultimate pH (p<0.05), and yellowness (p<0.01). The lightweight broilers exhibited higher (p< 0.001) blood loss and lower (p<0.05) cooking loss compared to the heavyweight broilers, regardless of the restraint method used and the duration of restraint. However, the interaction between the restraint method, duration of restraint, and body weight contributed to differences in pre-slaughter stress and meat quality. Therefore, the interaction between the restraint method and the duration of restraint affected the meat shear force, lightness (L*) and redness (a*).
Conclusion: The duration of restraint and body weight undoubtedly affect stress responses and meat quality of broiler chickens. Regardless of the duration of restraint and body weight, the cone restraint resulted in notably lower stress, lower meat water loss, and higher blood loss compared to shackling. Overall, the findings of this study showed that restraint method, duration of restraint, and body weight may affect the stress response and meat quality parameters in broilers and should be considered independently or interactively in future studies.

참고문헌 (Reference)

1 Berri C, "Variations in chicken breast meat quality : implications of struggle and muscle glycogen content at death" 46 : 572-579, 2005

2 Debut M, "Variation of chicken technological meat quality in relation to genotype and preslaughter stress conditions" 82 : 1829-1838, 2003

3 Lyon CE, "The location of wing restraints and the incidence of residual blood in broiler breast meat" 3 : 355-361, 1994

4 Nettle D, "The evolutionary origins of mood and its disorders" 22 : 712-721, 2012

5 Lambooij E, "The effects of captive bolt and electrical stunning, and restraining methods on broiler meat quality" 78 : 600-607, 1999

6 Hillebrand SJW, "The effects of alternative electrical and mechanical stunning methods on hemorrhaging and meat quality of broiler breast and thigh muscles" 75 : 664-671, 1996

7 Salwani MS, "Skeletal muscle proteome and meat quality of broiler chickens subjected to gas stunning prior slaughter or slaughtered without stunning" 14 : 375-381, 2016

8 Kannan G, "Shackling of broilers : effects on stress responses and breast meat quality" 38 : 323-332, 1997

9 SAS Institute, "SAS user’s guide. Version 8.2" SAS Institute Inc 1999

10 Dransfield E, "Relationship between muscle growth and poultry meat quality" 78 : 743-746, 1999

11 Honikel KO, "Reference methods for the assessment of physical characteristics of meat" 49 : 447-457, 1998

12 Abdulla NR, "Physico-chemical properties of breast muscle in broiler chickens fed probiotics, antibiotics or antibiotic–probiotic mix" 45 : 64-70, 2017

13 Hunt MC, "Meat color measurements" Purdue University 41-46, 1980

14 Yalçin S, "Interaction of transport distance and body weight on preslaughter stress and breast meat quality of broilers" 53 : 175-182, 2012

15 Zhu X, "High post-mortem temperature combined with rapid glycolysis induces phosphorylase denaturation and produces pale and exudative characteristics in broiler Pectoralis major muscles" 89 : 181-188, 2011

16 Kranen RW, "Hemorrhages in muscles of broiler chickens : the relationship among blood variables at various rearing temperature regimens" 75 : 570-576, 1996

17 Abdullah YA, "Growth performance, carcass and meat quality characteristics of different commercial crosses of broiler strains of chicken" 47 : 13-21, 2010

18 Hoving-Bolink AH, "Fiber area and capillary supply in broiler breast muscle in relation to productivity and ascites" 66 : 397-402, 2000

19 Lyasere OS, "Elevated levels of the stress hormone, corticosterone, cause ‘pessimistic’ judgment bias in broiler chickens" 7 : 6860-, 2017

20 Santiago HL, "Effects of strain, plane of nutrition, age at slaughter on performance and meat quality traits of broilers" 84 (84): 128-, 2005

21 Huang JC, "Effects of physical restraint and electrical stunning on plasma corticosterone, postmortem metabolism, and quality of broiler breast muscle" 92 : 5749-5756, 2014

22 Bouton PE, "Effect of ultimate pH upon the water-holding capacity and tenderness of mutton" 36 : 435-439, 1971

23 Berri CN, "Effect of selection for improved body composition on muscle and meat characteristics of broilers from experimental and commercial lines" 80 : 833-838, 2001

24 Schneider BL, "Effect of holding temperature, shackling, sex, and age on broiler breast meat quality" 91 : 468-477, 2012

25 Hermans EJ, "Dynamic adaptation of large-scale brain networks in response to acute stressors" 37 : 304-314, 2014

26 Gregory NG, "Duration of wing flapping in chickens shackled before slaughter" 121 : 567-569, 1987

27 Gao J, "Corticosterone alters meat quality by changing pre-and postslaughter muscle metabolism" 87 : 1609-1617, 2008

28 Liu R, "Contribution of nitric oxide and protein S-nitrosylation to variation in fresh meat quality" 144 : 135-148, 2018

29 Buzzard B, "Comparison of pig restraint, sampling methods, and analysis on blood lactate concentration" Kansas State University 409-414, 2012

30 Zhang W, "Breast meat quality of broiler chickens can be affected by managing the level of nitric oxide" 92 : 3044-3049, 2013

31 Debut M, "Behavioural and physiological responses of three chicken breeds to pre-slaughter shackling and acute heat stress" 46 : 527-535, 2005

32 Md. Shawkat Ali, "A Review: Influences of Pre-slaughter Stress on Poultry Meat Quality" 아세아·태평양축산학회 21 (21): 912-916, 2008