돼지고기육종의 방사선 유래 휘발성 물질 분석 = Irradiation-derrved Volatile Components in Irradiated Pork Meats

다국어 초록 (Multilingual Abstract)

Bacillus cereus KCTC 3674 produced at least two kinds of extracellular proteases. Two major bands of the protease activity with molecular weights of approximately 100 and 38 kDa were obtained after gelatin-SDS-PAGE. The production of extracellular proteases occurred during the logarithmic growth phase and was greatest when cultures reached the stationary growth phase. On the other hand, the growth of B. cereus KCTC 3674 and the production of its extracellular proteases were severely inhibited by the addition of protonophore carbonylcyanide m-chlorophenylhydrazone (CCCP). Thus, it was concluded that the growth of B. cereus KCTC 3674 and the production of its extracellular pretenses were strongly affected by a H+ electrochemical potential.

목차 (Table of Contents)

• Ⅰ. Introduction
• Ⅱ. Materials and Methods
• 2.1. Bacterial strain and growth conditions
• 2.2. Extracellular protease assay
• 2.3. Experimental system for the depletion of H+ electrochemical potential
• 2.4. Detection of protease activity on gelatin-SDS-PAGE
• Ⅲ. Results and Discussion
• 3.1. Production and properties of extracellular proteases
• 3.2. Effect of a H+ electrochemical potential on the production of extracellular