Paraquat로 처치한 백서에서 Melatonin과 수용성 비타민 E복합 투여가 지질과산화와 생존율에 미치는 영향 = The Effect of Combined Treatmens with Melatonin and Water-soluble Vitamin E in Lipid Peroxidation and Survival Rate after Paraquat Intoxication in Rats

다국어 초록 (Multilingual Abstract)

Objectives : Paraquat, an oxygen radical-generating agent, is a highly toxic compund for humans ans animals. This study was examined the effect of combined treatment of melatonin and Trolox^(®)(water-soluble analogue of α-tocopherol) in lipid peroxidation and mortality after paraquat intoxication in rats. Methods : Paraquat(20mg/kg) was injected i.p. into Spraque-Dawely rats. After the administration of paraquat, rats were treated with melatonin(10mg/kg), Trolox^(®)(100mg/kg), combined melatonin and Trolox^(®). All rats were killed by decapitation 24hours after paraquat administration and then examined the concentration of malonaldehye and 4-hydroxyalkenal (MDA + 4-HAE) as indices of lipid peroxidation in rat lung and liver. In a survival study, continous i.p. infusion of melatonin (50mg/kg/day) for 14 days using Alzet^(®) Osmotic pump (2ML2) and Trolox^(®)(100mg/kg/day) was injected i.p. after paraquat (20mg/kg) poisoning into rats for 14days. Results : Paraquat alone increased MDA + 4-HAE levels in lung, with this increase being reduced by melatonin and Trolox^(®), and also combined treatment with melatonin and Trolox^(®) completely reversed this effect. Paraquat at 20mg/kg did not induced any significant change in liver lipid peroxidation. In a result of survival analysis, percent survival was 100 % (8/8, control), 0 % (0/8, paraquat only), 62.5 % (5/8, paraquat + melatonin), 37.5 % (3/8, paraquat + Trolox^(®)), 75.0 % (6/8, paraquat + melatonin + Trolox^(®)) at 14 days after paraquat intoxication.
Conclusions : These results indicate that melatonin and Trolox^(®) protect lipid peroxidation and increase survival time in paraquat intoxicated rats. Combined treatment with melatonin and Trolox^(®) seems to have more survival benefit than single agent treatment in paraquat intoxication.