분산변이주 Archangium gephyra KYC5002의 자실체 발달과 포자 형성에 대한 정량적 분석 = Quantitative analysis of the fruiting body development and sporulation of the dispersion mutant Archangium gephyra KYC5002

다국어 초록 (Multilingual Abstract)

Fruiting body development and glycerol spore formation of the myxobacterium Archangium gephyra have been quantitatively analyzed using the KYC5002 strain, which grows in a dispersed manner in liquid media. KYC5002 formed many fruiting bodies that are smaller in size than those of the wild-type strain on various agar media and developed the most intact fruiting bodies on MMC agar plates composed of 10 mM 3-morpholinopropane- 1-sulfonic acid, 8 mM MgSO4, 0.015% casitone, and 3% agar.
Fruiting bodies developed when the cell suspension concentration was 5 × 108–5 × 109 cells/ml, but no fruiting bodies were formed at cell suspension concentrations higher or lower than that. The long and thin vegetative cells transformed into thick and short fruiting body spores in the fruiting bodies. Transformation into glycerol spores was possible at all time points in liquid culture, but it was achieved with high efficiency during the period of vigorous cell growth, yet with low efficiency during the stationary phase. The produced glycerol spores were morphologically similar to the fruiting body spores, and showed resistance to ultrasound and heat; however, its heat resistance was lower than that of the spores of the fruiting body.

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