Korean Society for Laboratory Medicine Practice Guidelines for the Molecular Diagnosis of Middle East Respiratory Syndrome During an Outbreak in Korea in 2015

다국어 초록 (Multilingual Abstract)

For two months between May and July 2015, a nationwide outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) occurred in Korea. On June 3, 2015, the Korean Society for Laboratory Medicine (KSLM) launched a MERS-CoV Laboratory Response Task Force (LR-TF) to facilitate clinical laboratories to set up the diagnosis of MERS-CoV infection. Based on the WHO interim recommendations, the Centers for Disease Control and Prevention of United States guidelines for MERS-CoV laboratory testing, and other available resources, the KSLM MERS-CoV LR-TF provided the first version of the laboratory practice guidelines for the molecular diagnosis of MERS-CoV to the clinical laboratories on June 12, 2015. The guidelines described here are an updated version that includes case definition, indications for testing, specimen type and protocols for specimen collection, specimen packing and transport, specimen handling and nucleic acid extraction, molecular detection of MERS-CoV, interpretation of results and reporting, and laboratory safety. The KSLM guidelines mainly focus on the molecular diagnosis of MERS-CoV, reflecting the unique situation in Korea and the state of knowledge at the time of publication.

참고문헌 (Reference)

1 Centers for Disease Control and Prevention, "interim patient under investigation (PUI) guidance and case definitions"

2 WHO, "WHO Laboratory Biosafety Manual"

3 Korea Center for Disease Control and Prevention, "The guideline for safe transportation of infectious substances"

4 WHO, "Novel coronavirus 2012: Interim recommendations for laboratory biorisk management"

5 Command and Control Center, "Ministry of Health, Kingdom of Saudi Arabia, The case definition for MERS-CoV infections approved by the ministry of health"

6 WHO, "Middle Ease respiratory syndrome coronavirus. Case definition for reporting to WHO. Interim case definition as of 14 July 2015"

7 WHO, "MERS-CoV in Republic of Korea at a glance as of 29 July 2015"

8 Central MERS-CoV Control Office, "MERS Statistics"

9 WHO, "Laboratory testing for Middle East Respiratory Syndrome Coronavirus. Interim guidance"

10 Zaki AM, "Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia" 367 : 1814-1820, 2012

11 Centers for Disease Control and Prevention, "Interim guidelines for collecting, handling, and testing clinical specimens from patients under investigation (PUIs) for Middle East Respiratory Syndrome Coronavirus (MERS-CoV) – Version 2.1"

12 Korea Centers for Disese Control and Prevention, "Instructions for the safe transport of infectious substances"

13 Corman VM, "Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction" 17 : 2012

14 Centers for Disease Control and Prevention, "CDC Laboratory Testing for Middle East Respiratory Syndrome Coronavirus (MERS-CoV)"

15 Corman VM, "Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections" 17 : 2012