B35 백서 신경모세포종 세포에서 KCI에 의한 탈분극이 일중 주기 관련 분자 CLOCK의 발현에 미치는 영향 = Regulation of the Circadian Gene CLOCK Expression by KCI Depolarization in B35 Rat Neuroblastoma Cells

다국어 초록 (Multilingual Abstract)

Objectives : To investigate the effects of KCl on regulation of circadian gene CLOCK expression, we observed whether induction of CLOCK is influenced by KCl depolarization in B35 rat neuroblastoma cells
Methods : B35 rat neuroblastoma cells were grown in Dulbecco's modified Eagle's medium supplemented with 10% FBS and 1% penicillin-streptomycin in a 37℃ humidified incubator with 5% CO₂. Inhibitors including cycloheximide and actinomycin D were pretreated 1 hour before treatment with 50mM KCl. Immunoblotting with anti-CLOCK antibody was done.
Results : CLCOK is induced by 50 mM KCl in B35 Rat Neuroblastoma cells, and a maximal induction in CLOCK level reached peak at 8 to 20 hours. The pretreatment of cycloheximide and actinomycin D prevented the induction of CLOCK by 50 mM KCl.
Conclusion : We suggest that KCl depolarization may play critical roles in several aspects of the circadian gene CLOCK expression.

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