Phosphorylation of Chicken Protein Tyrosine Phosphatase 1 by Casein Kinase II = Casein Kinase ll에 의한 닭의 protein tyrosine phosphatase 1의 인산화 및 효소활성의 조절

다국어 초록 (Multilingual Abstract)

CPTP1 is a nontransmembrane chickenprotein tyrosine phosphatase similar to human PTP1B (HPTP1B). To investigate whether the phosphatase activity of CPTP1 could be phosphoylated by casein kinase II. In vitro phosphorylation and phosphoamino acid analysis showed that both CPTP1 and HPTP1B were phosphorylated on serine and threonine residues by casein kinase II (CKII). Phosphorylation of CPTP1 by CKII resulted in an inhibition of its phosphatase activity in vitro. Endogenous CPTP1-like rat PTP1 was recongnized as a single protein of 51 kDa in rat adipocytes. But CKII did not interact with rat PTP1 in the insulin treated rat adipocytes. These results suggest that rat PTP1 is not implicated directly to CKII-induced signal transduction cascades.

목차 (Table of Contents)

• ABSTRACT
• INTRODUCTION
• MATERIAL AND MDTHODS
• Construction of expression vectors
• Expression and purification of GST fusion proteins in E.coli
• producion of anti-CPTP1 antibody
• Kinetic assay of CPTP1
• In vitro phosphorylation
• Phosphoamino acid analysis
• Preparation of rat adipocytes
• Immunoprecipitation and immunoblotting
• RESULTS AND DISCUSSION
• REFERENCES