Structure and Function of RSDA Gene Coding Raw-starch-digesting-amylase = RSDA 유전자의 분자구조와 기능

다국어 초록 (Multilingual Abstract)

The entire nucleotide sequence encoding the raw starch-digesting α-amylase gene(rsda), molecularly cloned from Bacillus circulans F-2 has been determined. Large preceding and following regions, comprising the presumed transcriptional and translational regulatory regions, were also sequenced. The probable coding region of the gene is 2508 base pairs(836 amino acids) long. The rsda sequence shows a open reading frame (ORF) with the translated molecular weight of about 96.727, which correspond to a molecular weight of about 93,000 and a presumed signal sequence of approximately 34 amino acids. The gene is preceded by the sequence resembling promoter for the B. subtilis sigma?? RNA polymerase and followed by the first codon of the gene. Homologous regions with other amylases were found. The N-terminal sequences of the mature proteins expressed in E. coli were identical to the 35 N-terminal sequences.