반응표면분석법을 이용한 새싹보리 마이크로웨이브 추출공정의 최적화 = Optimization of microwave-assisted extraction process of Hordeum vulgare L. by response surface methodology

다국어 초록 (Multilingual Abstract)

This study attempted to find optimum extract range of active ingredient for barley sprouts (Hordeum vulgare L.). Extracts from Hordeum vulgare L. were made by microwave extraction method and total polyphenol content (TPC), total flavonoid content (TFC), DPPH radical scavenging activity (DPPH) were measured with extract of Hordeum vulgare L.. Response surface methodology (RSM) was applied to a extraction process, and central composite design (CCD) was also used for this process to examine the optimum condition. Independent variables (Xn) are concentration of ethanol (X1: 0, 25, 50, 75, 100%), microwave power (X2: 60, 120, 180, 240, 300 W), extraction time (X3: 4, 8, 12, 16, 20 min). Dependent variables (Yn) are TPC (Y1), TFC (Y2), DPPH radical scavenging (Y3). It is formed by sixteen conditions to extract. The R2 value of dependent variables is ranged from 0.90 to 0.97 (p<0.05). Experiments values within the optimal range (40% of ethanol concentration, 120 W of microwave power, 18 min of extraction time) were 3.74 mg GAE/g (TPC), 3.00 mg RE/g (TFC), 35.43% (DPPH), respectively. Under the optimized conditions, predicted value showed no significant difference comparing with the experimental values.

참고문헌 (Reference)

1 김지수, "쑥부쟁이속 6종의 새싹채소 발아, 재배 및 저장에 미치는 환경요인의 영향" 한국원예학회 33 (33): 638-646, 2015

2 이은주, "생강 분획의 gingerols 분석 및 초임계 추출물의 항산화 효과" 한국식품과학회 43 (43): 469-474, 2011

3 전소윤, "새싹채소 및 쌈채소의 선호도 및 소비실태" 한국식품저장유통학회 19 (19): 783-791, 2012

4 박성은, "새싹보리를 첨가한 스트링치즈의 품질 특성" 한국식품영양과학회 46 (46): 841-847, 2017

5 인만진, "보리잎 분말차의 제조와 그 품질특성" 한국식품영양과학회 35 (35): 734-737, 2006

6 은청수, "보리싹 추출물의 항산화 및 항염증 활성" 한국생명과학회 26 (26): 537-544, 2016

7 정지은, "반응표면분석에 의한 진피 에탄올 추출물의 추출조건 최적화" 한국식품저장유통학회 18 (18): 755-763, 2011

8 이혜진, "반응표면분석에 의한 산수유 추출물의 추출조건 최적화" 한국식품영양과학회 41 (41): 390-395, 2012

9 이수정, "반응표면분석에 의한 개똥쑥의 항산화 활성을 위한 최적 추출 조건 확립" 농업생명과학연구원 47 (47): 103-114, 2013

10 김대익, "반응표면분석법을 이용한 야관문(Lespedeza cuneata) 기능성분의 에탄올 추출조건 최적화" 한국식품조리과학회 28 (28): 275-283, 2012

11 박대희, "반응표면분석법을 이용한 댕댕이 기능성성분의 마이크로웨이브추출조건 최적화" 한국식품저장유통학회 24 (24): 623-630, 2017

12 민들레, "반응표면분석법을 이용한 대추잎 항산화물질의 에탄올추출조건 최적화" 한국식품과학회 42 (42): 733-738, 2010

13 권중호, "마이크로파 추출공정에 의한 쥐깨풀 유용성분의 추출조건 최적화" 한국식품저장유통학회 12 (12): 617-623, 2005

14 이혜진, "마이크로웨이브 추출조건에 따른 참나무 겨우살이 추출물의 생리활성" 한국식품저장유통학회 18 (18): 72-78, 2011

15 주은영, "마이크로웨이브 추출조건에 따른 백지 추출물의 폴리페놀 함량과 항산화 작용" 한국식품저장유통학회 15 (15): 133-138, 2008

16 박은주, "마이크로웨이브 추출조건에 따른 동결 및 열풍 건조오미자 추출물의 항산화 특성" 한국식품과학회 45 (45): 667-674, 2013

17 이무열, "국화과 기생초, 각시취 및 저먼캐모마일 새싹채소의 발아, 재배 및 저장에 미치는 환경요인" 한국자원식물학회 22 (22): 136-144, 2009

18 Kang HM, "vegetables Sprouts, Fresh-cutting and processing" 555-558, 2013

19 Bang HR, "The effects of barley sprouts(Hordeum Vulgare L. )extract on bone metabolism in ovariectomized rats" Kyung Hee University 2017

20 Bezerra MA, "Response surface methodology(RSM)as a tool for optimization in analytical chemistry" 76 : 965-977, 2008

21 Kwon JH, "Optimization of microwave-assisted extraction under atmospheric pressure condition for soluble ginseng components" 32 : 117-124, 2000

22 Lee SB, "Optimization of extraction conditions for soluble ginseng components using microwave extraction system under pressure" 28 : 409-416, 1999

23 Mi-Jin Park, "Neuraminidase inhibitory activity of polyphenolic compounds from the barley sprouts (Hordeum vulgare L.)" 부산대학교 대학원 2013

24 Pare JRJ, "Microwave- assisted natural products extraction"

25 문정혜, "LED 광질에 따른 보리, 밀 새싹채소의 생육 특성" 공주대학교 대학원 2013

26 Lim SB, "Extraction yields of Hizikia fusiforme and Aloe vera Linne by supercritical carbon dioxide and antimicrobial activity of their extracts" 27 : 68-73, 1995

27 Lee SY, "Establishment of extraction conditions for effective components from Angelica gigas Nakai using microwave-assisted process" 29 : 442-447, 2000

28 Lee JH, "Effects of barley sprout and its compounds, hexacosanol and saponarin, on cholesterol and glucose metabolism" Korea University 2015

29 Byun AR, "Effects of a dietary supplement with barley sprout extract on blood cholesterol metabolism" 7-, 2015

30 Lee J, "Effects of a dietary supplement extracted from barley sprouts on cholesterol metabolism" Ewha Womans University 2013

31 Kim AR, "Effect of barley leaf ethanol extract on lipid metabolism in rats fed high cholesterol diet" 4 : 15-22, 2012

32 Kwak DH, "Determination of food safety in the process for the preparation of edible oil by the analysis of residual organic solvent" Seoul National University of Technology 2001

33 Lee YH, "Barley sprouts extract attenuates alcoholic fatty liver injury in mice by reducing inflammatory response" 8 : 440-, 2016

34 Han SY, "Antioxidative effects of the extract from barley sprout and application of the extracts to an edible oil and fat" Chonbuk National University 2011

35 Lee KS, "Analysis of consumer´s present use of sprout vegetables and quality characteristics of grain sourdough and barley sprouts" Catholic University 2015

36 Yoo KM, "A opinion on the growth & revitalization of health functional food market" 20 (20): 4-7, 2015

37 Seo MW, "21. vegetables Sprouts" 123-126, 2013