Penicillamine과 Corticosteroid가 백서피부창상 치유의 교원질 형성에 미치는 영향 = The Effects of Penicillamine and Corticosteroid on the Collagen Formation During the Repair of Skin Wound in Rats

다국어 초록 (Multilingual Abstract)

Ultimate response to tissue injury is repair by either parenchymal or connective tissue regeneration, and the most important component of dermal injury is collagen formation, which unfortunately results in to scar formation. Process of collagen biosynthesis has been studied extensively (ross, 1968; Heughan and Hunt, 1975). However. site of collagen formation, either intra or extra-cellular, is yet to be settled (Ross and Benditt, 1965; Hashimoto, 1967; Gothlin and Ericsson, 1970; Weinstock and Leblond, 1974).
Many attempts to minimize scar formation during the process of wound repair have been made, and several agents have been provided to inhibit collagen formation during the process of wound repair have been made, and several agents have been provided to inhibit collagen formation. corticosteroids are known to delay wound healing by inhibiting inflammatory response and collagen formation (Watts et al, 1965; Heughan and Hunt, 1975), and recently penicillamine is proved to inhibit collagen formation(Nimni and Bavetta, 1965; Heughan and Hunt, 1975).
Present study is aimed to elucidate effects of penicillamine and corticosteroids on collagen formation during healing of dermal injury by light and electron microscopic observations with aid of histochemical and cytochemical methods.
A total of 81 rats, 180-200gm, were divided into simple skin wound followed by either penicillamine or corticosteroid administration groups. Penicillamine was given subcutaneously in a dose of 10mg per animal per day and dexamethasone intramuscularly 0.6mg per kg per day. Animals were killed at various intervals, and the specimens were taken from the wound and processed for both light and electron microscopic examinations. For the light microscopic examinations, hematoxylin-eosin, Masson's trichrome, alcian-blue, and reticulin staining were made. For electron microscopic examinations, routine uranyl acetate and lead hydroxide staining, and periodic acid methenamine silver staining to demonstrate procollagen and collagen were made. Hitachi HU-11 E model electron microscope was used for the ultrastructural observations.