Synergistic effect of melamine in combination with cyanuric acid on urinary tract toxicity in rats

다국어 초록 (Multilingual Abstract)

The present study investigated the urinary tract toxicity of melamine alone or in combination with cyanuric acid in rats. Male rats were orally administered melamine alone (800 mg/kg) or melamine plus cyanuric acid (50 mg/kg each) for 3 consecutive days. Although melamine treatment did not show any significant difference in body weight, kidney or urinary bladder weight, serum blood urea nitrogen (BUN) or creatinine levels, it caused a decrease in urinary pH and mild histopathological alterations in the kidney and urinary bladder. In contrast, co-administration of melamine and cyanuric acid induced a decrease in body weight, an increase in kidneys and urinary bladder weights, and an elevation in serum BUN and creatinine levels, which were not observed in animals treated with melamine alone. Histopathological examination showed that the incidence and severity of histopathological lesions in the kidney and urinary bladder were much higher than those in the melamine group. Urinalysis revealed an increase in urine occult blood,leukocytes and protein and a decrease in urinary pH. These results indicate that concomitant administration of cyanuric acid synergistically potentiated the urinary tract toxicity induced by melamine in rats. In this study, we first demonstrated the synergistic toxic effects of melamine in combination with cyanuric acid on urinary bladder and urinalysis in rats.

참고문헌 (Reference)

1 Melnick RL, "Urolithiasis and bladder carcinogenicity of melamine in rodents" 72 : 292-303, 1984

2 World Health Organization, "Toxicological and Health Aspects of Melamine and Cyanuric Acid"

3 Organization for Economic Cooperation and Development, "Screening Information Data Set for Melamine, CAS No. 108-78-1"

4 Organization for Economic Cooperation and Development, "Screening Information Data Set for Isocyanuric acid, CAS No, 108-80-5"

5 Cook AM, "Ring cleavage and degradative pathway of cyanuric acid in bacteria" 231 : 25-30, 1985

6 Brown CA, "Outbreaks of renal failure associated with melamine and cyanuric acid in dogs and cats in 2004 and 2007" 19 : 525-531, 2007

7 Zhang X, "Melamineinduced infant calculi: a report on 24 cases and a 1-year follow-up" 38 : 391-395, 2010

8 Hau AK, "Melamine toxicity and the kidney" 20 : 245-250, 2009

9 McPheron T, "Melamine and cyanuric acid interaction may play part in illness from recalled pet food"

10 박동선, "Increased Nephrotoxicity after Combined Administration of Melamine and Cyanuric Acid in Rats" 한국실험동물학회 27 (27): 25-28, 2011

11 Dobson RL, "Identification and characterization of toxicity of contaminants in pet food leading to an outbreak of renal toxicity in cats and dogs" 106 : 251-262, 2008

12 Chen KC, "Evaluation of subchronic toxicity of pet food contaminated with melamine and cyanuric acid in rats" 37 : 959-968, 2009

13 Kim SH, "Effects of melamine on pregnant dams and embryo-fetal development in rats" 31 : 506-514, 2011

14 Lam CW, "Diagnosis and spectrum of melamine-related renal disease: Plausible mechanism of stone formation in humans" 402 : 105-155, 2009

15 Parry J, "China’s tainted infant formula sickens nearly 13,000 babies" 337 : a1802-, 2008

16 Puschner, B, "Assessment of melamine and cyanuric acid toxicity in cats" 19 : 616-624, 2007

17 Hammond BG, "A review of toxicology studies on cyanurate and its chlorinated derivatives" 69 : 287-292, 1986