Osteoblast Differentiation of Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Enhanced Cell Adhesion by Fibronectin = Osteoblast Differentiation of Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Enhanced Cell Adhesion by Fibronectin

다국어 초록 (Multilingual Abstract)

Mononuclear cells isolated from human umbilical cord blood were differentiated in to spindle shaped fibrous mesenchymal stem cells (MSCs) in DMEM with 10% fetal bovine serum. These progenitor cells were further examined for their ability to differentiate into osteoblasts by culturing them in osteogenic differentiation media (Stem Pro(R)). Calcium mineralization assay, alizarin red S, von kossa staining confirmed that MSCs from cord blood were capable of mineralization when they were cultured in osteogenic medium. The adhesion studies shown that MSCs have greater affinity towards fibronectin (FN) coated dishes, compared to albumin (Alb) coated dishes due to CD29and CD44 surface receptors.

참고문헌 (Reference)

1 A Alhadlaq, "Tissue-engineered neogenesis of humanshaped mandibular condyle from rat mesenchymal stem cells" 82 : 951-, 2003

2 G Pande, "The role of membrane lipids in regulation of integrin functions" 12 : 569-, 2000

3 DS Armilage, "The london cord blood bank: analysis of banking and transplantation outcome" 125 : 358-, 2004

4 K Bisanz, "Targeting ECM–integrin interaction with liposome-encapsulated small interfering rnas inhibits the growth of human prostate cancer in a bone xenograft imaging mode" 12 : 634-, 2005

5 JD Kretlow, "Review: mineralization of synthetic polymer scaffolds for bone tissue engineering" 13 : 927-, 2007

6 U Hersel, "RGD modified polymers: biomaterials for stimulated cell adhesion and beyond" 24 : 4385-, 2003

7 MS Friedman, "Osteogenic differentiation of human mesenchymal stem cells is regulated by bone morphogenetic protein-6" 98 : 538-, 2006

8 IC Bonzani, "Novel materials for bone and cartilage regeneration" 10 : 568-, 2006

9 MF Pittenger, "Multilineage potential of adult human mesenchymal stem cells" 284 : 143-, 1999

10 OK Lee, "Isolation of multipotent mesenchymal stem cells from umbilical cord blood" 103 : 1669-, 2004

11 A Vander, "Function and interactions of integrins" 305 : 285-, 2001

12 S Johansson, "Fibronectinintegrin interactions" 2 : d126-, 1997

13 J Sottile, "Fibronectin matrix assembly enhances adhesion-dependent cell growth" 111 : 2933-, 1998

14 RG Lebaron, "Extracellular matrix cell adhesion peptides: functional applications in orthopedic materials" 6 : 85-, 2000

15 JE Phillips, "Engineering graded tissue interfaces" 105 : 12170-, 2008

16 DMF Basle, "Effects of roughness, fibronectin and vitronectin on attachment, spreading, and proliferation of human osteoblast-like cells, saos-2, on titanium surfaces" 64 : 499-, 1999

17 K Chaipinyo, "Effects of growth factors on cell proliferation and matrix synthesis of low-density, primary bovine chondrocytes cultured in collagen I gels" 20 : 1070-, 2002

18 AS Goldstein, "Effect of adsorbed fibronectin concentration on cell adhesion and deformation under shear on hydrophobic surfaces" 59 : 665-, 2001

19 N Ogura, "Differentiation of the human mesenchymal stem cells derived from bone marrow and enhancement of cell attachment by fibronectin" 46 : 207-, 2004

20 J George, "Differentiation of mesenchymal stem cells into osteoblasts on honeycomb collagen scaffolds" 95 : 404-, 2006

21 DU Da, "Differential expression of stem cell mobilization-associated molecules on muti-lineage cells from adipose tissue and bone marrow" 89 : 267-, 2003

22 M Wang, "Composite scaffolds for bone tissue engineering" 2 : 80-, 2006

23 U Geibler, "Collagen type I coating of Ti6Al4V promotes adhesion of osteoblasts" 51 : 752-, 2000

24 JR Porter, "Bone tissue engineering: a review in bone biomimetics and drug delivery strategies" 25 : 1539-, 2009

25 KJL Burg, "Biomaterial developments for bone tissue engineering" 21 : 2347-, 2000