Bacillus thuringiensis 의 CryI 과 CytA 단백질의 형태와 용해도에 대한 숙주의 영향 = Effects of Host Cell on the Morphology and Solubility of CryI and CytA Protein of Bacillus thuringiensis

다국어 초록 (Multilingual Abstract)

The cryIB, truncated cryIB[cryIB(α)], cryIA(b), and cytA genes, encoding 135-, 89-, 131-, and 27-kDa proteins, respectively, from Bacillus thuringiensis were cloned into a shuttle vector pBES and expressed in E. coli and Bacillus species. The morphology and solubility in alkaline buffer of the insecticidal crystal proteins were investigated. Transformation of intact cells of E. coli and Bacillus species was achieved by electroporation. High field strength of 11.0 kV/㎝ and resistance of 129 ohms were required for efficient transformation of E. coli strains and 4.5 kV/㎝ and 48 ohms for Bacillus species. Strains of recombinant E. coli and Bacillus species produced the insecticidal crystal proteins and accumulated as the same bipyramidal and irregular structures as those of CryIB and IA(b) and CytA of B. thuringiensls, respectively. The insecticidal crystal proteins accumulated in recombinant E. coli wire smaller in size than those in recombinant Bacillus species. The solubility in alkaline buffer of the insecticidal crystal proteins of recombinant E. coli increased gradually as the pH increased, whereas in the case of Bacillus species the solubility increased gradually as the pH increased up to 9 and then the solubility increased greatly up to two times higher than that of E. coli proteins.