Theileria sergenti DNA probe 제작을 위한 기초 연구 = (A) Study for a construction of theileria sergento DNA probe

다국어 초록 (Multilingual Abstract)

This study was attempted to develope a method for detection of Theileria sergenti infection on the basis of hybridization of parasite DNA with a probe.
For construction of a T sergenti genomic library, T sergenti DNA was digested completely with Bam-HI and the fragments were ligated into the Bam-HI site of pUC-19 before transformation of Escherichia Coli strain JM83.
To detect clones containing the parasite's DNA sequences, a genomic DNA library of T sergenti constructed in pUC-19 was screened by cracking and Southern hybridization.
Seven colonies were chosen from 29 colonies which were screened by transformation of Escherichia coli strain JM83. Seven transformants were comfirmed from seven colonies by cracking. The sizes of transformants were about 5 Kb, 5.7 Kb, 4.3 Kb, 7.75 Kb, 7.85 Kb, 5.8 Kb, 3.8 Kb, respectively.
DNA inserts, T sergenti DNA, and bovine DNA were hybridized with radio-labelled T sergenti DNA. Two (pT_4, pT_1) of the seven inserts and T sergenti DNA reacted strongly but another 5 inserts and bovine DNA showed weak reaction.
All of the DNA inserts were not reaction, but T sergenti DNA were very weakly and bovine DNA were strongly reacted to hybridization with radio-labelled bovine DNA.
Therefore, we obtained total 7 T sergenti DNA fragments in this study.

목차 (Table of Contents)

• 목차
• 영문초록 = i
• 서론 = 1
• 재료 및 방법 = 3
• T sergenti 원충 = 3
• DNA의 분리 = 3
• 제한효소처리 = 4
• Ligation = 4
• Transformation = 5
• Cracking = 5
• Mini-scale Preparation of plasmid DNA = 6
• Transfer and Hybridization of DNA = 7
• 결과 = 10
• 고찰 = 16
• 결론 = 19
• 참고문헌 = 20