瓦松의 알콜 抽出物이 家兎의 腎機能에 미치는 影響 = Effect of the Water Fraction Separated from Alcohol Extract of Orostachys malacophyllus F. on the Renal Functions of Rabbits

다국어 초록 (Multilingual Abstract)

Orostachys malacophyllus F. has been applied in Chinese medicine as well as in folk remedy. Its chemical composition and pharmacological actions have not reported yet. But, sometimes, it was used in treatment for the stomach or lung cancer.
This study was attempted to investigate the effects of the water fraction separated from alcohol extract of the plant on the renal functions of rabbits. After cannulation of carotid artery, jugular vein and ureters were prepared for the recording of blood pressure, the infusion of the water extract(0.005%) or the hypotonic saline(0.45%), and the collection of the urine, respectively. The hypotonic saline was infused to hydrate the rabbits, and the mixed solution of hypotonic saline, PAH, and inulin was infused to measure the glomerular filtration rate(GFR) and renal plasma flow (RPF). After the hydration, the changes of urine volume were obsereved during the infusion of the water fraction for 1 hour, and during the infusion of hypotonic saline for 80min. And the concentration of PAH or inulin in the urine and blood was measured by the method of Smith and the method of Schreiner, electrolyte concentration by flamephotometer, and the osmolar conentration by osmometer, respectively.
The results were summarized as follows:
1. Urine volume, GFR, and RPF were decreased significantly by the infusion of water fraction, and thery were not recovered to control values after the exchange to hypotonic saline.
2. Filtration fraction was increased by water fraction.
3. Water fraction decreased the plasma clearances of electrolytes and the osmolar substances, and increased the negative free water clearance.
4. Water fraction increased the reabsorptive rate(%)of potassium, and decreased the excretory amount of Na or K. And it was decreased the ratio of Na/K excretion.
From the above results, it was suggested that water fraction has the antidiuretic effect and its mechanism is primarily caused by the change of the renal hemodynamics.