Development of topical natural based film forming system loaded propolis from stingless bees for wound healing application

다국어 초록 (Multilingual Abstract)

Purpose Propolis extract is an extraction of a resinous mixture of honey bees produced by mixing saliva and beeswax with exudate gathered from tree. The prominent pharmacological activities of propolis extract are antioxidant, antimicrobial, fungicidal, antiviral, antiulcer, immune-stimulating, and cytostatic, which are very suitable for topical formulation.
Methods From its various pharmacological activities, propolis extract was formulated in film forming system using modified tamarind seed gum as a solution/gel/film forming agent. To obtain the optimum seed gum concentration of the film forming system (solution to film), various tamarind concentrations and solvent ratios were evaluated.
Results The formulation of 7.5% of modified tamarind seed gum with 1% PEG400 dissolved in 55%:45% of water:ethanol provided fast drying time (4.36 min) and proper rheological property for spaying application. The propolis extract loaded formulation could inhibit Staphylococcus aureus and Staphylococcus epidermidis. Moreover, the product satisfaction evaluation was excellent (3.8 from 5).
Conclusion The results indicate the possibility of introducing modified tamarind seed gum and propolis extract as a component in the development of film forming systems and increasing the effectiveness of antibacterial on the skin.

참고문헌 (Reference)

1 World Medical Association, "World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects" 310 : 2191-2194, 2013

2 Huanbutta K, "Use of seed gums from Tamarindus indica and Cassia fistula as controlled-release agents" 13 : 398-408, 2018

3 Dhiman S, "Transdermal patches : a recent approach to new drug delivery system" 3 : 26-34, 2011

4 Karki S, "Thin films as an emerging platform for drug delivery" 11 : 559-574, 2016

5 Mezger TG, "The rheology handbook: for users of rotational and oscillatory rheometers" Vincentz Network GmbH & Co KG 2006

6 Frederiksen K, "The potential of polymeric film-forming systems as sustained delivery platforms for topical drugs" 13 : 349-360, 2016

7 Joseph J, "Tamarind seed polysaccharide: a promising natural excipient for pharmaceuticals" 6 : 270-278, 2012

8 Jalil MAA, "Stingless bee honey, the natural wound healer : a review" 30 : 66-75, 2017

9 Cohen J, "Statistical power analysis for the behavioral sciences" Academic Press 2013

10 Klykken P, "Silicone film-forming technologies for health care applications" 1 : 1-8, 2004

11 Cohen MP, "Sample size considerations for multilevel surveys" 73 : 279-287, 2005

12 Kurt A, "Rheology of film-forming solutions and physical properties of differently deacetylated salep glucomannan film" 5 : 175-184, 2019

13 Chen CH, "Rheological and physical characterization of film-forming solutions and edible films from tapioca starch/decolorized hsian-tsao leaf gum" 23 : 2132-2140, 2009

14 Pavot W, "Review of the satisfaction with life scale. Assessing well-being" Springer 101-117, 2009

15 Ammar H, "Rapid pain relief using transdermal film forming polymeric solution of ketorolac" 18 : 1005-1016, 2013

16 Shariatinia Z, "Polysaccharide carriers for drug delivery" Elsevier 639-684, 2019

17 Balouiri M, "Methods for in vitro evaluating antimicrobial activity : A review" 6 : 71-79, 2016

18 Vongsak B, "In vitro cytotoxicity of Thai stingless bee propolis from Chanthaburi orchard" 14 : 741-747, 2017

19 Stepanovic S, "In vitro antimicrobial activity of propolis and synergism between propolis and antimicrobial drugs" 158 : 353-357, 2003

20 Drago L, "In vitro antimicrobial activity of a novel propolis formulation(Actichelated propolis)" 103 : 1914-1921, 2007

21 Saingam W, "Formulation development of topical film formimg spray from Piper nigrum L" 42 (42): 219-222, 2018

22 Kathe K, "Film forming systems for topical and transdermal drug delivery" 12 : 487-497, 2017

23 Bajaj H, "Film forming gels : a review" 7 : 2085-2091, 2016

24 Moyon R, "Film forming complex, cosmetic composition comprising it and their use as peel-off film mask"

25 Gohel MC, "Fabrication of modified transport fluconazole transdermal spray containing ethyl cellulose and Eudragit ® RS100 as film formers" 10 : 684-691, 2009

26 Mori NM, "Fabrication and characterization of film-forming voriconazole transdermal spray for the treatment of fungal infection" 55 : 41-51, 2017

27 Faradilla RF, "Effect of polyethylene glycol(PEG)molecular weight and nanofillers on the properties of banana pseudostem nanocellulose films" 205 : 330-339, 2019

28 Touitou E, "Drug delivery across the skin" 2 : 723-733, 2002

29 Huanbutta K, "Development of tamarind seed gum as dry binder in formulation of diclofenac sodium tablets" 13 : 863-874, 2016

30 Sangnim T, "Design and characterization of clindamycin-loaded nanofiber patches composed of polyvinyl alcohol and tamarind seed gum and fabricated by electrohydrodynamic atomization" 13 : 450-458, 2018

31 MÈndez-Vilas A, "Current research, technology and education topics in applied microbiology and microbial biotechnology" Formatex Research Center 2010

32 Campos JF, "Antimicrobial, antioxidant, anti-inflammatory, and cytotoxic activities of propolis from the stingless bee Tetragonisca fiebrigi(Jataí)" 2015 : 1-11, 2015

33 Wiegand I, "Agar and broth dilution methods to determine the minimal inhibitory concentration(MIC)of antimicrobial substances" 3 : 163-175, 2008

34 Ramsey R, "A review of the effects of humidity, humectants, and surfactant composition on the absorption and efficacy of highly water-soluble herbicides" 82 : 162-175, 2005