흰쥐 뇌의 중심편도핵에서의 substance P 및 leucine-enkephalin 함유구조에 대한 전자현미경적 연구 = Distribution and ultrastructure of substance P and leucineenkephalinimmunoreactive structures in the central amygdaloid nucleus of the rat

다국어 초록 (Multilingual Abstract)

In order to investigate the distribution and ultrastructure of substance P(SP) and leucine-enkephalin(Leu-Enk) immunoreactive structures in the central amygdaloid nucleus, brains of the rats were processed for peroxidase-antiperoxidase technique.
The results obtained were as follows;
SP immunoreactive neurons were found in the medial zone and immunoreactive axon terminals were heaviest in the lateral capsular zone. The immunoreactive neurons were round or oval in shape (diameter, 15-20㎛) and had two tor three proximal dendrites.
SP immunoreactive neurons were characterized by a relatively thin rim of cytoplasm that surrounded an unlabelled nucleus. The immunoreactive products were found throughout the cytoplasm of the cell bodies and dendrites. Non-reactive axon terminals made synaptic contacts more frequently with the soma than with the dendrits of the immunoreactive neuron.
Most of the synaptic terminals on SP immunoreactive somas were of type Ⅱ. The immunoreactive dendrites made both of type Ⅰ and Ⅱ synaptic contacts; the latter were more common.
SP immunoreactive axon terminals contained numerous small (40-50㎚) clear vesicles and a few large(100-150n), dense cored vesicles. The reaction product characteristically filled the core of the large granulated vesicle and surrounded the outer membrane of the small clear vedicles.
The majority of axon terminals displaying SP immunoreactivity formed symmetrical synapses with dendrites and, less frequently, with somas of nonreactive neurons.
Leu-Enk immunoreactive neurons and axon terminals were located within central and capsular zones. The immuno-reactive neurons were round or oval shape(diameter, 17-24㎛) and had threeof four proximal dendrites.
Compared to the ultrastructural characteristics of SP immunoreactive neurons, those of Leu-Enk immunoreactive neurons showed similar morphological features except that type Ⅱ synaptic contacts were more common between immunoreactive dendrites and nonreactive axon terminals.