Anther and Pollen Ontogeny of Tea Plant (Camellia sinensis)

국문 초록 (Abstract)

본 연구는 중국종(C. sinensis var. sinensis)과 아샘종(C.sinensis var. assamica)의 차[Camellia sinensis (L.) O. Kuntze] 품종에서 약 및 화분의 발생 과정에 대해 상세하게 분석하기 위해 수행되었다. 꽃의 발육단계에 따라 채취한 꽃을 고정액에 고정하고 염색한 후 약 및 화분의 발생 과정 을 현미경하에서 분석하였다. 약은 네 개의 화분낭으로 이 루어져 포원세포, 화분모세포, 사분자기, 일핵성 소포자 및 이핵성 성숙 화분으로 발달하는 화분 발생과정을 나타내 었다. 성숙한 화분립은 원형에서 삼각형에 이르는 형태로 화분 외막과 내막으로 둘러 쌓여 있었다. 약벽의 발생학적 발달은 식물에서의 일반적인 형태를 따라 외피, 하피성 내 피, 중간 유조직층, 융단조직층으로 분화되었다. 약의 열개 는 길이 방향의 열개선을 따라 이루어지며, 이핵성 화분이 방출되었다. 후기 풍선상의 꽃 발달 단계 성숙 화분은 성공 적인 수분 및 수정의 전제가 되는 양질의 활력 및 발아력을 나타내었다. 본 연구결과는 중국종 및 아샘종의 차 품종은 매우 유사한 꽃의 발달단계에 따른 약 및 화분의 발생학적 관계를 가지고 있음을 나타내었다.

다국어 초록 (Multilingual Abstract)

The study was conducted to provide comprehensive details on anther and pollen ontogeny of the tea plant [Camellia sinensis (L.) O. Kuntze] based on the varieties of China type (C. sinensis var. sinensis) and Assam type (C. sinensis var. assamica). Young and mature flowers at different progressive developmental stages were collected. The anther and pollen ontogeny was determined by optical microscopy after fixation and staining. The results revealed tetrasporangiate anthers with a various ontogenetic sequences of pollen (archesporial cells, microspore mother cells, tetrahedral tetrads, uninucleate microspore, and two-celled mature pollen grain). Mature pollen grains were spherical to triangular in shape, tricolporate, and enclosed with the exine and intine. The ontogenetic sequence of anther wall development followed the basic type and was composed of an epidermal layer, an endothelial layer, two middle layers, and a glandular type tapetum. Anther dehiscence occurred by means of longitudinal slits and the mature pollen grains liberated at a bicellular stage. Mature pollen at the late balloon phenophase stage of flowers possessed considerable quality by means of viability and germinability which is a prerequisite for successful pollination and fertilization. The results indicated that an analogous anther and pollen ontogenesis at the selected progressive developmental stages of flowers in China type and Assam type tea varieties showed a good correlation with the precise stages of the anther and pollen ontogeny.

목차 (Table of Contents)

• Introduction
• Materials and Methods
• Results and Discussion

참고문헌 (Reference)

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