Development of Fermentation Process for PLA-degrading Enzyme Production by a New Thermophilic Actinomadura sp. T16-1

다국어 초록 (Multilingual Abstract)

The fermentation process for a poly (L-lactide) (PLA)-degrading enzyme production by a newly isolate of thermophilic PLAdegrading
Actinomadura sp. T16-1 was investigated. The strain produced 33.9 U/mL of enzyme activity after cultivation at
50°C under shaking of 150 rpm for 96 h in a medium consisting of (w/v) 0.05% PLA film, 0.2% gelatin, 0.4% (NH4)2SO4,
0.4% K2HPO4, 0.2 % KH2PO4, and 0.02% MgSO4 ⋅ 7H2O. The optimal concentration of PLA film and gelatin obtained by response
surface methodology (RSM) for the highest production of PLA-degrading enzyme was 0.035% (w/v) and 0.238%
(w/v), respectively. Under these conditions, the model predicted 40.4 U/mL of PLA-degrading activity and the verification of
the optimization showed 44.6 U/mL of PLA-degrading enzymatic activity in the flasks experiment. The maximum PLAdegrading
activity reached 150 U/mL within 72 h cultivation in the 3-L airlift fermenter

다국어 초록 (Multilingual Abstract)

The fermentation process for a poly (L-lactide) (PLA)-degrading enzyme production by a newly isolate of thermophilic PLAdegrading
Actinomadura sp. T16-1 was investigated. The strain produced 33.9 U/mL of enzyme activity after cultivation at
50°C under shaking of 150 rpm for 96 h in a medium consisting of (w/v) 0.05% PLA film, 0.2% gelatin, 0.4% (NH4)2SO4,
0.4% K2HPO4, 0.2 % KH2PO4, and 0.02% MgSO4 ⋅ 7H2O. The optimal concentration of PLA film and gelatin obtained by response
surface methodology (RSM) for the highest production of PLA-degrading enzyme was 0.035% (w/v) and 0.238%
(w/v), respectively. Under these conditions, the model predicted 40.4 U/mL of PLA-degrading activity and the verification of
the optimization showed 44.6 U/mL of PLA-degrading enzymatic activity in the flasks experiment. The maximum PLAdegrading
activity reached 150 U/mL within 72 h cultivation in the 3-L airlift fermenter

참고문헌 (Reference)

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