Temporal and spatial impact of Spartina alterniflora invasion on methanogens community in Chongming Island, China

다국어 초록 (Multilingual Abstract)

Methane production by methanogens in wetland is recognized as a significant contributor to global warming. Spartina alterniflora (S. alterniflora), which is an invasion plant in China’s wetland, was reported to have enormous effects on methane production. But studies on shifts in the methanogen community in response to S. alterniflora invasion at temporal and spatial scales in the initial invasion years are rare. Sediments derived from the invasive species S. alterniflora and the native species Phragmites australis (P. australis) in pairwise sites and an invasion chronosequence patch (4 years) were analyzed to investigate the abundance and community structure of methanogens using quantitative real-time PCR (qPCR) and Denaturing gradient gel electrophoresis (DGGE) cloning of the methyl-coenzyme M reductase A (mcrA) gene. For the pairwise sites, the abundance of methanogens in S. alterniflora soils was lower than that of P. australis soils. For the chronosequence patch, the abundance and diversity of methanogens was highest in the soil subjected to two years invasion, in which we detected some rare groups including Methanocellales and Methanococcales. These results indicated a priming effect at the initial invasion stages of S. alterniflora for microorganisms in the soil, which was also supported by the diverse root exudates. The shifts of methanogen communities after S. alterniflora invasion were due to changes in pH, salinity and sulfate. The results indicate that root exudates from S. alterniflora have a priming effect on methanogens in the initial years after invasion, and the predominate methylotrophic groups (Methanosarcinales) may adapt to the availability of diverse substrates and reflects the potential for high methane production after invasion by S. alterniflora.

참고문헌 (Reference)

1 Smith, D.L., "Vegetation-mediated changes in microclimate reduce soil respiration as woodlands expand into grasslands" 85 : 3348-3361, 2004

2 Wei, D., "Uncertainty and dynamics of natural wetland CH4 release in China: Research status and priorities" 154 : 95-105, 2017

3 Fontaine, S., "The priming effect of organic matter: a question of microbial competition?" 35 : 837-843, 2003

4 Kendall, M.M., "The order methanosarcinales" Springer 2006

5 Bonin, A.S., "The order methanobacteriales" Springer 2006

6 Liu, D.Y., "The impact of dissolved organic carbon on the spatial variability of methanogenic archaea communities in natural wetland ecosystems across China" 96 : 253-263, 2012

7 Narihiro, T., "The impact of aridification and vegetation type on changes in the community structure of methane-cycling microorganisms in Japanese wetland soils" 75 : 1727-1734, 2011

8 Sakai, S., "The family Methanocellaceae" Springer 2014

9 Yang, L.H., "The extraction, isolation and identification of exudates from the roots of Flaveria bidentis" 13 : 105-114, 2014

10 Zheng, Y., "Sulfate reducer and sulfur oxidizer respond differentially to the invasion of Spartina alterniflora in estuarine salt marsh of China" 99 : 182-190, 2017

11 Smith, J.M., "Structure and function of methanogens along a short-term restoration chronosequence in the Florida everglades" 73 : 4135-4141, 2007

12 Wang, J.X., "Spartina alterniflora, alters ecosystem DMS and CH4, emissions and their relationship along interacting tidal and vegetation gradients within a coastal salt marsh in Eastern China" 167 : 349-359, 2017

13 Li, B., "Spartina alterniflora invasions in the Yangtze River estuary, China: An overview of current status and ecosystem effects" 35 : 511-520, 2009

14 Yang, W., "Spartina alterniflora invasion alters soil microbial community composition and microbial respiration following invasion chronosequence in a coastal wetland of China" 6 : 26880-, 2016

15 Fernandez-de Cordova, M.L., "Solid phase spectrophotometric microdetermination of iron with ascorbic acid and ferrozine" 357 : 44-49, 1997

16 McCulley, R.L., "Soil respiration and nutrient cycling in wooded communities developing in grassland" 85 : 2804-2817, 2004

17 Marschner, P., "Soil and plant specific effects on bacterial community composition in the rhizosphere" 33 : 1437-1445, 2001

18 Yuan, J.J., "Shifts in methanogen community structure and function across a coastal marsh transect: effects of exotic Spartina alterniflora invasion" 6 : 18777-, 2016

19 Kotsyurbenko, O.R., "Shift from acetoclastic to $H_2$-dependent methanogenes is in a West Siberian peat bog at low pH values and isolation of an acidophilic Methanobactetium strain" 73 : 2344-2348, 2007

20 Kuzyakov, Y., "Review of mechanisms and quantification of priming effects" 32 : 1485-1498, 2000

21 Yang, W., "Response of the soil microbial community composition and biomass to a short-term Spartina alterniflora, invasion in a coastal wetland of eastern China" 408 : 1-14, 2016

22 Zhang, Y., "Response of methane emission to invasion of Spartina alterniflora and exogenous N deposition in the coastal salt marsh" 44 : 4588-4594, 2010

23 Ashelford, K.E., "New screening software shows that most recent large 16S rRNA gene clone libraries contain chimeras" 72 : 5734-5741, 2006

24 Norton, U., "Moisture pulses, trace gas emissions and soil C and N in cheatgrass and native grass-dominated sagebrushsteppe in Wyoming, USA" 40 : 1421-1431, 2008

25 Jonasson, S., "Microbial biomass C, N and P in two arctic soils and responses to addition of NPK fertilizer and sugar: Implications for plant nutrient uptake" 106 : 507-515, 1996

26 Lu, Y., "Methanogenic responses to exogenous substrates in anaerobic rice soils" 32 : 1683-1690, 2000

27 Galand, P.E., "Methanogen communities in a drained bog: Effect of ash fertilization" 49 : 209-217, 2005

28 Murrell, J.C., "Methane, microbes and models: fundamental understanding of the soil methane cycle for future predictions" 15 : 2395-, 2013

29 Nahlik, A.M., "Methane emissions from tropical freshwater wetlands located in different climatic zones of Costa Rica" 17 : 1321-1334, 2011

30 Ganzert, L., "Metha nogenic communities in permafrost-affected soils of the Laptev Sea coast, Siberian Arctic, characterized by 16S rRNA gene fingerprints" 59 : 476-488, 2007

31 Rooney-Varga, J.N., "Links between archaeal community structure, vegetation type and methanogenic pathway in Alaskan peatlands" 60 : 240-251, 2007

32 Chen, J.Q., "Invasive Spartina and reduced sediments: Shanghai's dangerous silver bullet" 1 : 79-84, 2008

33 Liao, C.Z., "Invasion of Spartina alterniflora enhanced ecosystem carbon and nitrogen stocks in the Yangtze Estuary, China" 10 : 1351-1361, 2007

34 Wang, R.Z., "Impacts of Spartina alterniflora invasion on the benthic communities of salt marshes in the Yangtze Estuary, China" 36 : 799-806, 2010

35 Yang, W., "Impacts of Spartina alterniflora invasion on soil organic carbon and nitrogen pools sizes, stability, and turnover in a coastal salt marsh of eastern China" 86 : 174-182, 2016

36 Chmura, G.L., "Global carbon sequestration in tidal, saline wetland soils" 17 : 2003

37 Fan, L., "Functional equivalence and evolutionary convergence in complex communities of microbial sponge symbionts" 109 : E1878-E1887, 2012

38 Conrad, R., "Functional and structural response of the methanogenic microbial community in rice field soil to temperature change" 11 : 1844-1853, 2009

39 She, C.X., "Factors regulating community composition of methanogens and sulfatereducing bacteria in brackish marsh sediments in the Min River estuary, southeastern China" 181 : 27-38, 2016

40 Zeleke, J., "Effects of Spartina alterniflora invasion on the communities of methanogens and sulfate-reducing bacteria in estuarine marsh sediments" 4 : 243-, 2013

41 Bu, N.S., "Effects of Spartina alterniflora invasion on soil respiration in the Yangtze River Estuary, China" 10 : e0121571-, 2015

42 Cao, P., "Distribution and diversity of archaeal communities in selected Chinese soils" 80 : 146-158, 2012

43 Bai, J.H., "Depth-distribution patterns and control of soil organic carbon in coastal salt marshes with different plant covers" 6 : 34835-, 2016

44 Chung, C.H., "Creation of Spartina plantations for reclaiming Dongtai, China, tidal flats and offshore sands" 23 : 135-150, 2004

45 Fei Xia, "Communities of ammonia oxidizers at different stages of Spartina alterniflora invasion in salt marshes of Yangtze River estuary" 한국미생물학회 53 (53): 311-320, 2015

46 Zhang, Y., "Changes in soil organic carbon dynamics in an eastern chinese coastal wetland following invasion by a $C_4$ plant Spartina alterniflora" 42 : 1712-1720, 2010

47 Tong, C., "Changes in pore-water chemistry and methane emission following the invasion of Spartina alterniflora into an oliogohaline marsh" 63 : 384-396, 2017

48 Parkes, R.J., "Changes in methanogenic substrate utilization and communities with depth in a salt-marsh, creek sediment in southern England" 96 : 170-178, 2012

49 Chen, X.P., "Changes in methane emission and methanogenic and methanotrophic communities in restored wetland with introduction of Alnus trabeculosa" 17 : 181-189, 2016

50 Zhang, J.H., "Bioassay and identification of root exudates of three fruit tree species" 49 : 257-261, 2007

51 Buyantuyev, A., "A Space-For-Time (SFT) substitution approach to studying historical phenological changes in urban environment" 7 : e51260-, 2012