고려인삼에서 Malate Dehydrogenase 유전자의 분리 및 분석

다국어 초록 (Multilingual Abstract)

Malate dehydrogenase is a ubiquitous enzyme in plants, involving in a range of metabolic processes depending on its subcellular location. A malate dehydrogenase (PgMDH) cDNA was isolated and characterized from the root of Panax ginseng C. A. Meyer. The deduced amino acid sequence of PgMDH showed high similarity with the NAD-dependent mitochondrial malate dehydrogenase from Glycinemax (P17783), Eucalyptus gunnii (P46487), and Lycopersicon esculentum (AAU29198). And the segment of a malate dehydrogenase gene was amplified through RT-PCR. The expression of PgMDH was increased after treatments of chilling, salt, UV, cadmium or copper treatment.

참고문헌 (Reference)

1 Kumari M, "Trascriptomic responses to aluminum stress in roots of Arabidopsis thaliana" 279 (279): 339-357, 2008

2 Hara S, "Thioredoxin-h1 reduces and reactives the oxidized cytosolic malate dehydrogenase dimmer in higher plants" 281 : 32065-32071, 2006

3 Ok Tae Kim, "Thermotolerant Transgenic Ginseng (Panax ginseng C.A. Meyer)by Introducing Isoprene Synthase Gene throughAgrobacterium tumefaciens-mediated Transformation" 한국약용작물학회 15 (15): 95-99, 2007

4 Sweetlove LJ, "The impact of oxidative stress on Arabidopsis mitochondria" 32 : 891-904, 2002

5 Thompson JD, "The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools" 24 : 4876-4882, 1997

6 이성우, "Shading Effect of Different Colored Polyethylene net on Seeding Growth ofPanax ginseng C. A. Meyer" 한국약용작물학회 14 (14): 113-116, 2006

7 Thimm O, "Response of Arabidopsis to iron deficiency stress as revealed by microarray analysis" 1257 : 1030-1043, 2001

8 Verniquet F, "Rapid inactivation of plant aconitase by hydrogen peroxide" 276 : 643-648, 1991

9 Moller IM, "Plant mitochondria and oxidative stress: Electron transport, NADPH turnover, and metabolism of reactive oxygen species" 52 : 561-591, 2001

10 Morris PC, "Osmotic stress and abscisic acid regulate the expression of the Em gene of wheat" 190 : 625-630, 1990

11 Scheibe R, "Malate valves to balance cellular energy supply" 120 : 21-26, 2004

12 Gietl C, "Malate dehydrogenase isoenzymes: cellular locations and role in the flow of metabolites between the cytoplasm and cell organelles" 1100 : 217-234, 1992

13 Frishman D, "MIPS: a database for genomes and protein sequences" 28 : 37-40, 2000

14 Kumar S, "MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment" 5 : 150-163, 2004

15 Zimmermann P, "GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox" 136 : 2621-2632, 2004

16 MCAlister-Henn L, "Evolutionary relationships among the malate dehydrogeases" 13 : 178-181, 1988

17 Tejera NA, "Effect of some abiotic factors on the biological activity of Gluconacetobacter diazotrophicus" 95 : 528-535, 2003

18 Beechmans S, "Demonstration of physical interactions between consecutive enzymes of the citric acid cycle and of the aspartate-malate shuttle. A study involving fumarase, malate dehydrogenase, citrate cynthase and aspartate aminotransferase" 117 : 517-535, 1981

19 Felsenstein J, "Confidence limit on phylogenies: an approach using the bootstrap" 39 : 783-791, 1985

20 Jiang Y, "Comprehensive transcriptional profiling of NaCl-stressed Arabidopsis roots reveals novel classes of responsive genes" 6 : 25-25, 2006

21 Ocheretina O, "Cloning and sequence analysis of cDNAs encoding plant cytosolic malate dehydrogenase" 199 : 145-148, 1997

22 Altschul SF, "Basic local alignment search tool" 215 : 403-410, 1990

23 Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue" 15 : 473-497, 1962