치자의 HMC-1 비만세포에 대한 항염증효과

다국어 초록 (Multilingual Abstract)

Abstract


Anti-inflammatory effect of Gardenia Jasminoides extract (GJ) in HMC-1 human mast cell

Moon, Jihong
Dept. of Korean Medicine
Graduate School
Kyung Hee University
Seoul, Korea
Supervised by Prof. Ko, Seong-Gyu, K.M.D., Ph.D.

Objective: In this study, the anti-inflammatory effects of Gardenia Jasminoides were investigated in HMC-1 human mast cell. To identify which mechanism is involved in the anti-inflammatory mechanism, we investigated the inhibitory effect of GJ on PMA and Ionomycin-induced inflammation.

Methods: The effect of GJ was analyzed by RT-PCR, ELISA and Western blotting in PMA and Ionomycin-activated HMC-1, human mast cells.

Results: We found that GJ significantly inhibited the phosphorylation of NF-κB, ERK, JNK, and P38. In addition, GJ decreased nuclear translocation of NF-κB and the mRNA expression of IL-6, IL-8 and IL-17A. GJ also suppreseed the expression of pro-inflammatory cytokines such as IL-6 and TNF-α.

Conclusion: These results suggest that GJ has an anti-inflammatory effect through the phosphorylation of the NF-κB and MAPK proteins, thereby decreasing nuclear translocation of NF-κB and production of pro-inflammatory cytokines in HMC-1 mast cell.

Key words: Gardnia Jasminoides; anti-inflammatory; HMC-1; mast cell;

목차 (Table of Contents)

• I. 서 론 1
• II. 실험 재료 및 방법 4
• 1. 약물의 조제 4
• 2. 시약 5
• 3. 세포 배양 6
• 4. 생존율 평가 7
• 5. western blot 실험 8
• 6. Reverse Transcription-PCR 9
• 7. ELISA 10
• 8. 통계처리 11
• I. 결 과 12
• 1. 치자의 HMC-1 비만세포의 생존률에 미치는 영향 12
• 2. HMC-1 비만세포에서 NF-kappaB, MAPKs 의 인산화 억제 효과 14
• 3. HMC-1 비만세포에서 치자의 NF-kappaB의 핵 내 이동 억제효과 16
• 4. HMC-1 비만세포에서 치자의 IL-6, IL-8 와 IL-17A mRNA 억제효과 18
• 5. HMC-1 비만세포에서 치자의 IL-6와 TNF-α 생성 억제 효과 20
• III. 고 찰 22
• IV. 결 론 25
• 참 고 문 헌 26
• ABSTRACT 2