동백나무(Camellia japonica) 잎 추출물의 비만세포에서의 탈과립 억제 및 염증성 사이토카인 억제효과

다국어 초록 (Multilingual Abstract)

This study investigated the effects of leaf extract of Camellia japonica (LECJ) on antigen-stimulated degranulation in mast cells and its mechanism of action in cell lines. It was identified that the inhibitor activation in the examination of β-hexosaminidase, inflammatory cytokines and Syk kinase activation upon which LECJ were collected in the prime of antioxidant activity were 1/10 concentration lower than the patent published in 2007 and the paper published 2008. In the case of preparing LECJ, the inhibition of the concentration was 10 times greater than the start as reported in the existing paper published in 2007 and the patent published 2008. The effects of LECJ verified it as an effective material for cosmetic use. In the study, the antigen-stimulated activation of mast cells, the inhibition of degranulation on LECJ and its mechanism of action, LECJ exhibited an inhibitory effect on degranulation in mast cells. Also, the activation of signaling pathways in antigen-stimulated mast cells depends initially on the interaction of FceRI on the downstream
activation of Syk and other tyrosine kinases. This study showed that degranulation in antigen-stimulated mast cells and the secretion of inflammatory cytokines decreased in a concentration dependent manner.
These results showed that the LECJ in cell line is effective for its anti-allergic activity. In the case of LECJ, the study showed that the effects of degranulation, the effects of the expression and secretion of inflammatory cytokines, and the directly inhibiting activation of signaling molecules such as adaptors.

참고문헌 (Reference)

1 최완수, "호장근추출물 및 그의 제조방법과 알레르기 질환의 치료제로서 그의 용도" 건국대학교 산학협력단 2008

2 이선영, "피토라이트를 포함하는 미백 화장료 조성물"

3 김기호, "피부 수렴효과를 갖는 동백꽃 추출물을 함유하는화장료조성물"

4 이경록, "식물 추출물을 함유한 여드름 피부용 저자극 화장료 조성물"

5 김미경, "블루베리 잎 추출물의 항산화 및 항염증 효과" 한국인체미용예술학회 15 (15): 109-120, 2014

6 유선희, "백량금 열매 추출물이 항산화 및 항염작용에 미치는 영향" 한국인체미용예술학회 14 (14): 197-208, 2013

7 송현숙, "망초 에탄올추출물의 항비만 및 항염증활성에 관한 연구" 한국인체미용예술학회 18 (18): 231-241, 2017

8 김정환, "둥근마 에탄올 추출물의 항염증 효과" 한국인체미용예술학회 17 (17): 91-102, 2016

9 배용태, "동백나무 추출물을 유효성분으로 함유하는 노화 또는 암 예방 또는치료용 조성물"

10 최완수, "동백나무 추출물을 유효성분으로 하는 항 알레르기 조성물"

11 D. H. Chu, "The syk family of protein tyrosine kinases in T-cell activation and development" 165 : 167-180, 1998

12 M. K. Church, "The human mast cell" 99 : 155-160, 1997

13 H. Kihara, "Src homology 2 domains of Syk and Lyn bind to tyrosine-phosphorylated subunits of the high affinity IgE receptor" 269 : 22427-22432, 1994

14 M. A. Beaven, "Signal transduction and human disease" John Wiley and Sons Inc. 307-355, 2003

15 J. H. Lee, "Rubiae radix suppresses the activation of mast cells through the inhibition of Syk kinase for anti-allergic activity" 58 : 503-512, 2006

16 R. P. Siraganian, "Protein tyrosine kinase Syk in mast cell signaling" 38 : 1229-1233, 2002

17 B. O. Lim, "Polygoni cuspidati radix inhibits the activation of syk kinase in mast cells for antiallergic activity" 232 : 1425-14313, 2007

18 J. H. Lee, "Phospholipase D2 acts as an essential adaptor protein in the activation of Syk in antigen-stimulated mast cells" 108 : 956-964, 2006

19 S. J. Galli., "New concepts about the mast cells" 328 : 257-265, 1993

20 C. Zhang, "Mitogen activated protein (MAP) kinase regulates production of tumor necrosis factor-alpha and release of arachidonic acid in mast cells. Indications of communication between p38 and p42 MAP kinases" 272 : 13397-13402, 1997

21 A. M. Gilfillan, "Integrated signalling pathways for mast-cell activation" 6 : 218-230, 2006

22 D. C. Seldin, "Homology of the rat basophilic leukemia cell and the rat mucosal mast cell" 82 : 3871-3875, 1985

23 V. Parravinci, "Fyn kinase initiates complementary signals required for IgE-dependent mast cell degranulation" 3 : 741-748, 2002

24 G. Qian, "Differentiation of rhizome et radix Polygoni cuspidati from closely related herbs by HPLC fingerprinting" 54 : 1179-1186, 2006

25 K. Onodera, "Camellianoside, a novel anti-oxidant glycoside from the leaves of Camellia japonica" 70 : 1995-1998, 2006

26 J. H. Lee, "Camellia japonica suppresses immunoglobulin E-mediated allergic response by the inhibition of Syk kinase activation in mast cells" 38 (38): 794-804, 2008

27 J. H. Lee, "Camellia japonica suppresses immunoglobulin E-mediated allergic response by the inhibition of Syk kinase activation in mast cells" 38 : 794-804, 2008

28 S. F. Kemp, "Anaphylaxis:a review of causes and mechanisms" 110 : 341-348, 2002

29 T. K. Hung., "A Handbook of the Composition and Pharmacology of Common Chinese Drugs" China Medical Science and Technology 1222-1226, 1994