엘크사슴의 냉동정액 생산과 인공수정 = Frozen - Semen Production and Artificial Insemination in EIK Deer ( Cervus Elaphus canadensis )

다국어 초록 (Multilingual Abstract)

To establish the system for frozen-semen production, oestrus synchronization, and artificial insemination (AI) in Elk deer, the characteristics of frozen-semen and conception rate by artificial insemination were observed. Semen were collected from 2 heads of elk (Cervus Elaphus canadensis) sire by an electroejaculator and diluted to 0.9- 1.1 X 10^9 sperms/straw with the extender containing glycerol, hydroxymethyl amino methane, citric acid, fructose, and 20% egg yolk. The diluted semen were chilled at 4℃ for 3-4 hours, prefrozen at -140㎏ for 30-40 minutes, and preserved at liquid nitrogen (-196℃). The motility, viability, and abnormality of frozen-thawed semen were 62.4-70.5%, 73.7-75.3% and 6.8-7.3%, respectively. Oestrus was synchronized in 48 heads of hind with progesterone- impregnated intravaginal devices (CIDRs). The CIDRs were removed on Day 14, and 10-250 IU pregnant mare`s serum gonadotrophin (PMSG) were given to each hind. Oestrus exhibition rate was 95.8% (46/48). Forty-six hinds, showing oestrus, were artificially inseminated with frozen-thawed semen 60-62 hours after PMSG injection. Pregnancy was detected by ultrasonography at 40 days after Al. The overall conception rate was 78.3% (36/46). These results indicate that the system for frozen-semen production and AI established in this study may be adapted to deer industry.