LPS로 자극된 RAW 264.7 세포에서 암대극 추출물의 항염증 효과 = Anti-Inflammatory Activity of Euphorbia jolkini Extract in Lipopolysaccharide-Stimulated RAW264.7 Cells

다국어 초록 (Multilingual Abstract)

The Euphorbia jolkini (J11) is traditional medicinalplant, has been used for treatment diabetes, flu, toothache,etc. Recently studies shown that J11 extract has been evaluatedto anti-oxidant, anti-bacterial, and anti-melanogenesis.
However, Anti-inflammatory effect of J11 extract has notbeen reported yet. In this study, we studied the effects of J11extract on anti-inflammatory effect in lipopolysaccharide(LPS)-stimulated RAW 264.7 cells. We also studied the effectof J11 extract on expression of protein in RAW 264.7 cells.
The cells were treated with J11 extract, ranging between 25and 100 μg/mL. The results indicated that J11 extract dramaticallydecreased nitric oxide (NO) and prostaglandin 2 (PGE2)production in the cells without any cytotoxicity. In addition,J11 extract strongly inhibited expression of pro-inflammatorycytokines such as interleukin (IL)-6 and interleukin (IL)-1β.
Moreover, western blot result showed that the J11 extractinhibited inducible nitric oxide synthase (iNOS) and cyclooxygenase(COX-2) expression in a does-dependent manner.
Therefore, this study suggest that J11 extract may be appliedfor potential source as a natural anti-inflammatory agent.

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