Agrobacterium tumefaciens 유래 인디칸 분해활성을 갖는 β-glucosidase 의 분리와 특성분석

다국어 초록 (Multilingual Abstract)

Indican (indoxyl-β-D-glucoside) is a colorless natural compound and can be used as a precursor for the production of indigo. This production step only require an enzyme, β-glucosidase, that readily screened from microbial resource by using selective media supplemented with indican as a sole carbon source. Agrobacterium tumefaciens was well grown in this media and thus presumed to produce a related enzyme. The corresponding gene, encoding a protein with a calculated molecular mass of 51 kDa, was cloned and overexpressed as MBP fusion proteins. The purified enzyme was determined to be a dimer and showed the maximum activity for indican at pH 7.0 and 40℃. The kinetic parameters for indican, Km and Vmax, were determined to be 1.4 mM and 373.8 μM/min/mg, respectively. The conversion yield of indican into indigo using this enzyme was about 1.7-1.8 folds higher than that of previously isolated enzyme from Sinorhizobium meliloti. Additionally, this enzyme was able to hydrolyze various β-1,4 glycoside substrates.

목차 (Table of Contents)

• Abstract
• 1. 서론
• 2. 재료 및 방법
• 3. 결과 및 고찰
• 4. 결론
• References

참고문헌 (Reference)

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