Murine Melanoma B16 세포주의 항암제감수성 시험에 관한 실험적 연구 = Chemosensitivity test of Murin Melanoma B16 to Anticancer Drugs using Tetrazolium-based colorimetric Assay and Dye exclusion Assay

다국어 초록 (Multilingual Abstract)

Anticancer drug sensitivity assays were performed using cotorimetric[3-(4.5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide(MTT)] assay and dye exclusion assay <DEA> on cell line. Comparison of the MTT assay with DEA is essential prior to its use in clinical screening study.
Murine melanoma B16 cell lines were exposed to bleomycin, CDDP, Vinblastine for one hour(2.0×10^4 viable cells/well) and then cultured for four days in MEM containing 10% bovine serum for dye exclusion assay. In [MTT] assay, cells to be tested were exposed for one hour and continuously(5.0×10^3 viable cells/well). Four Hour before the culture end, added the MTT on media measured the absorbance with an ELISA reader at a wavelength of 490㎚.
The results were as follows ;
1. Cancer cells were sensitive to bleomycin, CDDP at all concentrations and bleomycin revealed relatively good cytotoxicity than that of CDDP in [DEA] assay(P<0.05).
2. In [MTT] assay, cancer cells were sensitive to high concentration of all drygs and medium concentration of vinblastine for 1 hour exposure(P<0.01).
3. In [MTT] assay, cancer cells were sensitive to medium and high concentration of all drugs and low concentration of VINB for vinblastine continuous exposure(P<0.01).
4. In the comparision of chemosensitivity test between [DEA] and [MTT] assay, the result was correlated(γ=0.99, P<0.05).