광분해성 캡 아날로그를 활용한 mRNA 발현 조절 연구 = Photocleavable 5’-Cap Analogues for Light-Controlled mRNA Expression

다국어 초록 (Multilingual Abstract)

Messenger RNA (mRNA) has become a key agent for preventing infectious diseases since the COVID-19 pandemic. In particular, modification of mRNA has been attempted by many different groups, and research is currently underway by modifying several different positions. This study introduces a method for synthesizing a material that can control translation efficiency by introducing a PhotoCleavable material into the mRNA Cap O6 position. This approach offers a novel method for light-controlled mRNA translation and suggests its potential as an effective approach for mRNA-based therapies. In this study, by synthesizingcap analogues and nucleotide precursors such as dinucleotide cap analogues, GMP-imidazolide, and GTP that can be controlled by specific ultraviolet rays, it is expected that the system can be activated at specific points or locations in the translation process, allowing for more precise control than existing systems. These compounds can be readily applied to the general and efficient production of mRNA using in vitro transcription. In conclusion, this study aims to open up new possibilities for mRNA translation control and lay the foundation for diverse biological and therapeutic applications.

목차 (Table of Contents)

• 초록 Abstract ii
• 1.서론 1
• 2. 실험방법 6
• 2.1 일반사항 6
• 2.2 광분해성 nucleoside 물질의 합성 7
• 2.3 광분해성 nucleotide 물질의 합성 13
• 2.4 UV test 26
• 2.5 mRNA 전사, 형질주입 27
• 3. 결과 및 토의 29
• 4. 결 론 33
• 5. 참고문헌 37
• 6. 분석자료 42