Angiotensin I-Converting Enzyme Inhibitory Activity of Sulfated Chitooligosaccharides with Different Molecular Weights = Angiotensin I-Converting Enzyme Inhibitory Activity of Sulfated Chitooligosaccharides with Different Molecular Weights

다국어 초록 (Multilingual Abstract)

The angiotensin I converting enzyme (ACE) inhibitory effects of three kinds of sulfated chitooligosaccharides (SCOS), relatively higher molecular weight SCOS (HMWSCOS, 5-10 kDa), medium molecular weight SCOS (MMWSCOS, 5-3 kDa), and lower molecular weight SCOS (LMWSCOS, 3-1 kDa), respectively. The MMWSCOS exhibited the higher inhibition activity with the IC50 value of 0.25mg/mL than other sulfated chitooligasaccharides (MMWSCOS: 0.775mg/mL and LMWSCOS: 0.325 mg/ml). Furthermore, all SCOSs showed no cytotoxicity on human embryonic lung fibroblast cell line (MRC-5) and Lineweaver-Burk plots suggest that MMWSCOS and LMWSCOS acts as non-competitive inhibitor to inhibit ACE. Therefore, these results exhibited that substitution of the hydrogen atom at the C-6 position of pyranose residue by the sulfate group promotes ACE inhibitory effects of COS and they would be beneficial ingredients for nutraceuticals and pharmaceuticals against hypertension and related diseases.