Fabrication of a Nanoplasmonic Chip to Enhance Neuron Membrane Potential Imaging by Metal-Enhanced Fluorescence Effect

다국어 초록 (Multilingual Abstract)

Optical imaging is a useful tool to acquire neural activities because of its high spatial resolution, and various voltage indicators were developed to image membrane potential of neurons. Voltage sensitive dyes (VSDs) are one of them but their signal-to-noise ratio (SNR) is so low that enhancing SNR of VSD has become important. In this study, we investigated the metal-enhanced fluorescence (MEF) effect on VSD imaging by fabricating nanoplasmonic resonance chip using gold nanorods (GNRs). To amplify the fluorescence signal we used polyelectrolyte layers to control the distance between metal nanoparticles and fluorophore. Cultured rat hippocampal neurons and di-8-ANEPPS, a widely used VSD, were used to test the nanoplasmonic resonance chip, and the maximum level of fluorescence signal was obtained when nine layers of polyelectrolyte spacer were used. The nanoplasmonic resonance chip with GNR showed the possibility of the improvement in voltage imaging of neurons and is expected to enhance the availability of neuronal activity imaging in the future.

참고문헌 (Reference)

1 Koh, B., "Visible to near-infrared fl uorescence enhanced cellular imaging on plasmonic gold chips" 12 : 457-465, 2016

2 Mei, Z., "Surface-plasmon-coupled fluorescence enhancement based on ordered gold nanorod array biochip for ultrasensitive DNA analysis" 89 : 633-639, 2017

3 Nik, B., "Preparation and growth mechanism of gold nanorods (NRs) using seed-mediated growth method" 15 : 1957-1962, 2003

4 Ray, K., "Polyelectrolyte layer-bylayer assembly to control the distance between fl uorophores and plasmonic nanostructures" 19 : 5902-5909, 2007

5 Darvill, D., "Plasmonic fl uorescence enhancement by metal nanostructures: Shaping the future of bionanotechnology" 15 : 15709-15726, 2013

6 Lu, M., "Plasmonic enhancement of cyanine dyes for near-infrared light-triggered photodynamic/photothermal therapy and fl uorescent imaging" 28 : 445710-, 2017

7 Kralj, J. M., "Optical recording of action potentials in mammalian neurons using a microbial rhodopsin" 9 : 90-95, 2012

8 Camposeo, A., "Metal-enhanced nearinfrared fl uorescence by micropatterned gold nanocages" 9 : 10047-10054, 2015

9 Bondre, N., "Metal-enhanced fl uorescence based calcium detection: greater than 100-fold increase in signal/noise using Fluo-3 or Fluo-4 and silver nanostructures" 152 : 82-87, 2011

10 Tawa, K., "In situ sensitive fl uorescence imaging of neurons cultured on a plasmonic dish using fl uorescence microscopy" 6 : 20010-20015, 2014

11 Peterka, D. S., "Imaging voltage in neurons" 69 : 9-21, 2011

12 Gong, Y., "High-speed recording of neural spikes in awake mice and fl ies with a fl uorescent voltage sensor" 350 (350): 1361-1366, 2015

13 Lu, Y., "High-density silver nanoparticle fi lm with temperature-controllable interparticle spacing for a tunable surface enhanced Raman scattering substrate" 5 : 5-9, 2005

14 Liu, B., "High performance, multiplexed lung cancer biomarker detection on a plasmonic gold chip" 26 : 7994-8002, 2016

15 Lee, J. W., "Gold nanostarmediated neural activity control using plasmonic photothermal eff ects" 153 : 59-69, 2018

16 Theodorou, I. G., "Gold nanostar substrates for metalenhanced fl uorescence through the fi rst and second near-infrared windows" 29 : 6916-6926, 2017

17 Pang, J., "Gold nanodisc arrays as near infrared metalenhanced fl uorescence platforms with tuneable enhancement factors" 5 : 917-925, 2017

18 Decher, G., "Fuzzy nanoassemblies: toward layered polymeric multicomposites" 277 (277): 1232-1237, 1997

19 Wang, J., "Fluorophoregold nanoparticle complex for sensitive optical biosensing and imaging" 23 : 095501-, 2012

20 Yang, H., "Fluorescence plasmonic enhancement of FITC labeled PS nanoparticles coupled to silver island fi lms" 55 : 5387-5392, 2016

21 Tawa, K., "Fluorescence microscopy imaging of cells with a plasmonic dish integrally molded" 55 : 03DF12-, 2016

22 Tang, S., "Fast kinetics of calcium signaling and sensor design" 27 : 90-97, 2015

23 Zhou, L., "Enhancement of immunoassay’s fl uorescence and detection sensitivity using three-dimensional plasmonic nano-antenna-dots array" 84 : 4489-4495, 2012

24 Park, J. E., "Emerging plasmonic nanostructures for controlling and enhancing photoluminescence" 8 : 4696-4704, 2017

25 Scanziani, M., "Electrophysiology in the age of light" 461 : 930-939, 2009

26 Yoo, S., "Electro-optical neural platform integrated with nanoplasmonic inhibition interface" 10 : 4274-4281, 2016

27 Feng, A. L., "Distance-dependent plasmonenhanced fluorescence of upconversion nanoparticles using polyelectrolyte multilayers as tunable spacers" 5 : 1-10, 2015

28 Mock, J. J., "Distance-dependent plasmon resonant coupling between a gold nanoparticle and gold fi lm" 8 : 2245-2252, 2008

29 Akbay, N., "Distance-dependent metalenhanced intrinsic fl uorescence of proteins using polyelectrolyte layer-by-layer assembly and aluminum nanoparticles" 116 : 10766-10773, 2012

30 Smetters, D., "Detecting action potentials in neuronal populations with calcium imaging" 18 : 215-221, 1999

31 Chen, Y., "Dependence of fl uorescence intensity on the spectral overlap between fl uorophores and plasmon resonant single silver nanoparticles" 7 : 690-696, 2007

32 Yuste, R., "Dendritic spines as basic functional units of neuronal integration" 375 : 682-684, 1995

33 Anker, J. N., "Biosensing with plasmonic nanosensors" 7 : 442-453, 2008

34 Wang, J., "Aspect ratio dependence of the enhancement of fl uorescence intensity by gold nanobipyramids for cancer cell imaging and photodynamic therapy" 28 : 075602-, 2018

35 Hochbaum, D. R., "All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins" 11 : 825-833, 2014

36 Piatkevich, K. D., "A robotic multidimensional directed evolution approach applied to fl uorescent voltage reporters" 14 : 901-901, 2018

37 Zhang, B., "A plasmonic chip for biomarker discovery and diagnosis of type 1 diabetes" 20 : 948-953, 2014

38 Xu, J., "A gold nanoparticle-based fl uorescence sensor for high sensitive and selective detection of thiols in living cells" 75 : 1-7, 2016