Mitochondrial haplotype distribution and phylogenetic relationship of an endangered species Reeve's turtle (Mauremys reevesii) in East Asia

다국어 초록 (Multilingual Abstract)

This study was examined to reveal haplotype distribution and phylogenetic relationship using mitochondrial DNA CYTB gene sequences of Reeve’s turtle (Mauremys reevesii) of East Asia. CYTB sequences of Reeve’s turtles were divided into 6 haplotypes (Hap01–Hap06). Chinese turtles were found in Hap01, Hap02, Hap04, and Hap05, and Hap01 was the highest frequency of 85.0%. Korean Turtles were found in Hap01, Hap03, Hap04, and Hap05, and Hap03 was the highest frequency of 52.1%. Although there was no haplotype which includes only the CYTB sequence exclusive for Reeve’s turtles of Korea, since no CYTB sequence of China was found in Hap03, it would be possible that Hap03 turtles of Korea are separated from those of China. The haplotypes of Reeve’s turtles of East Asia were monophyletic, which indicated that they had been evolved from a single maternal lineage, but went through local evolution after geographical migration and isolation in East Asia.

참고문헌 (Reference)

1 오홍식, "제주도에 이입된 황소개구리(Rana catesbeiana)와 붉은귀거북(Trachemys scripta elegans)의 서식실태 및 관리방안" 한국환경생태학회 21 (21): 311-317, 2007

2 김수련, "남생이 서식처 복원을 위한 서식처 모형 연구" 한국환경복원기술학회 16 (16): 115-125, 2013

3 van Dijk PP, "Turtles of the world, 2012 update:annotated checklist of taxonomy, synonymy, distribution, and conservation status" 5 : 243-328, 2012

4 Saitou N, "The neighbor-joining method: a new method for reconstructing phylogenetic trees" 4 : 406-425, 1987

5 장민호, "The Status of Distribution for Native Freshwater Turtles in Korea, with Remarks on Taxonomic Position" 한국환경생물학회 30 (30): 151-155, 2012

6 Lee JCI, "Species identification using the cytochrome b gene of commercial turtle shells" 3 : 67-73, 2009

7 National Institute of Biological Resources, "Research report of proliferation and restoration of Reeve’s turtle (Chinemys reevesii)" National Institute of Biological Resources 15-18, 2012

8 Clayton DA, "Replication of animal mitochondrial DNA" 28 : 693-705, 1982

9 National Institute of Biological Resources, "Red data book of endangered amphibians and reptiles in Korea" National Institute of Biological Resources 2011

10 Brown WM, "Rapid evolution of animal mitochondrial DNA" 76 : 1-967, 1979

11 Cheung SM, "Quantifying the Asian turtle crisis: market surveys in southern China, 2000e2003" 16 : 751-770, 2003

12 Chen TH, "Population status and distribution of freshwater turtles in Taiwan" 44 : 261-266, 2010

13 Honda M, "Phylogeny of the Eurasian freshwater turtles of the genus Mauremys Gray 1869 (Testudines), with special references to a close affinity of Mauremys japonica with Chinemys reevesii" 40 : 195-200, 2002

14 Suzuki D, "Origin of Japanese populations of Reeves’ pond turtle, Mauremys reevesii (Reptilia: Geomydidae), as inferred by a molecular approach" 10 : 237-249, 2011

15 Birky CW, "Organelle gene diversity under migration, mutation, and drift: equilibrium expectation, approach to equilibrium, effects of heteroplasmic cells, and comparison of nuclear genes" 121 : 613-627, 1989

16 IUCN (International Union for the Conservation of Nature), "Mauremys reevesii. The IUCN red list of threatened species. version 2015"

17 Tamura K, "MEGA6: molecular evolutionary genetics analysis version 6.0" 30 : 2-725, 2013

18 Barth D, "Is Chinemys megalocephala FANG, 1934a valid species? New insights based on mitochondrial DNA sequence data" 38 : 213-232, 2002

19 Xia X, "Interspecific hybridization between Mauremys reevesii and Mauremys sinensis: evidence from morphology and DNA sequence data" 10 : 6716-6724, 2011

20 Suzuki D, "Hybridization between Mauremys japonica and Mauremys reevesii inferred by nuclear and mitochondrial DNA analyses" 48 : 445-454, 2014

21 Lovich JE, "Geographic variation in the Asiatic Turtle Chinemys reevesii (Gray) and the status of Geoclemys grangeri Schmidt" 19 : 238-245, 1985

22 Fujii R, "Genetic and morphological assessments of hybridization between two nonnative Geoemydid turtles, Mauremys reevesii and Mauremys mutica, in North central Japan" 13 : 191-201, 2014

23 National Institute of Environmental Research, "Ecological guide book of herpetofauna in Korea" National Institute of Environmental Research 2011

24 Librado P, "DnaSP v5: a software for comprehensive analysis of DNA polymorphism data" 25 : 1451-1452, 2009

25 Fong JJ, "DNA evidence for the hybridization of wild turtles in Taiwan: possible genetic pollution from trade animals" 11 : 2061-2066, 2010

26 Chen TH, "Current status and utilization of the chelonians in Taiwan" 2 : 45-51, 2000

27 Song JY, "Current status and distribution of reptiles in the Republic of Korea" 25 : 124-138, 2007

28 Thompson JD, "CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties, and weight matrix choice" 22 : 4673-4680, 1994

29 CITES (Convention on International Trade in Endangered Species)., "Appendices I, II, and III"

30 Bickham JW, "An annotated list of modern turtle terminal taxa with comments on areas of taxonomic instability and recent change" 4 : 173-199, 2007

31 Kaiser H, "A widely traveled turtle: Mauremys reevesii (Testudines: Geoemydidae) in Timor-Leste" 3 : 093-096, 2010

32 Kimura M, "A simple method for estimating evolutionary rates of base substitutions through comparable studies of nucleotide sequences" 16 : 111-120, 1980

33 Lau CH, ". Genetic diversity of Asian water buffalo (Bubalus bubalis): mitochondrial DNA D-loop and cytochrome b sequence variation" 29 : 253-264, 1998