肝吸蟲感染에 따른 血淸 IgE와 血液內好酸球의 變動樣相에 관한 硏究 = Observation on the serum IgE level and eosinophil counts in Clonorchiasis

다국어 초록 (Multilingual Abstract)

The present study aimed to clarify the influence of single or repeated infection of Clonorchis sinensis on the IgE level and eosinophil counts of rabbit, and to determine the relationship between degree of infection and eosinophil counts or IgE level in the patients infected with C. sinensis.
Various numbers of metacercariae isolated from flesh of the second intermediate host, Pseudorasbora parva, were directly introduced to the rabbits.
Single infection group was divided into heavy, moderate and light infection groups, in which 2,000, 500 and 50 metacercariae of C. sinensis were administered respectively. Repeated infection group was also divided into heavy, moderate and light infection groups, in these cases 200, 100 and 10 metacercariae were fed biweekly for five times respectively.
Infection was confirmed by detection of the eggs of C. sinensis in feces five weeks after primary infection. Whole blood and sera were collected prior to infection, and on 1,3,5,7, and 9 weeks after infection from the inner marginal vein of rabbit's ear respectively. As a control, whole blood and sera were also sampled from the non-infected rabbits. Total eosinophil counts, differential count, and IgE levels were examined.
On the other hand, a total of 50 patients infected with C. sinensis was examined their degree of infection (EPG), total eosinophil counts and differential counts of white blood cells of 50 c. sinensis infected patients were exanined. Forty-six sera were sampled from patients infected with C. sinensis and the serum IgE levels were evaluated. In analysis of serum IgE level, Phadebas IgE PRIST® radioimmunoassay technique was applied.
The results are as follows:
1. Total eosinophil counts of repeated infection group of rabbits were significantly higher than that of single infection group. Total eosinophil counts of non-infected rabbits showed ranges of 0~65/㎣ blood during 9 weeks of examination. In the infected rabbits of heavy infection group, total eosinophil counts increased rapidly and reaching to peak on the 5th week after infection. These values maintained until the end of examination in 9th week (140/㎣ in single and 250/㎣ in repeated group). However, total eosinophil counts of light and modertate infection did not show rapid increase. On the contrary they increased slightly and continuously until 9 weeks after infection. Similar results were obtained from the differential counts of eosinophil.
2. In the examination of the patients infected with C. sinensis correlation between EPG and eosinophil count was recongnized. Coefficient of correlation was, r=0.61, rank correlation r=0.700, and regression equation was Yc(total eosinophil counts)=0.04χ (EPG)+372.
3. Total serum IgE levels in the patients infected with C. sinensis showed remarkably elevated value. mean values were 1,167 IU/㎖±1,172, but correlation with degree of infection was not significant statistically. Influences of repeated infection of C. sinensis on the IgE level were not able to investigate, because IgE was not detected in Phadebas IgE PRIST® using rabbit as an experimental animal.