Expression and Localization of Aquaporins in Benign Prostate Hyperplasia and Prostate Cancer

다국어 초록 (Multilingual Abstract)

The aquaporin (AQP) families of water channels are intrinsic membrane proteins that facilitate selective water and small solute movement across the plasma membrane. The purposes of this study were to determine the expression and localization of AQPs in benign prostatic hyperplasia and prostate cancer. Prostatic tissue was collected from patients with benign prostatic hyperplasia or prostate cancer by transurethral resection of the prostate. The expression and cellular localization of the AQPs were determined in the human prostate by Western blot and immunohistochemistry. AQP1,3, and 9 were expressed in the human prostate. Western blot analysis revealed bands at 28-36 kDa for the AQP1, 3, and 9 proteins. Of these proteins, AQP3 and 9 were expressed in the epithelium. Immunolabeling showed that AQP1 was mainly expressed in the capillaries and venules of the prostate, AQP9 was expressed in the cytoplasm of the epithelium, and AQP3 was mainly associated with the plasma membrane of the prostatic epithelium. Only AQP3 expression was localized in the cell membrane, and expressed AQP3 was translocated to the cytoplasm in prostate cancer. The epithelium in the human prostate expresses AQP3 and 9 proteins, and the capillaries and venules of the prostate express AQP1. Characterizing or modifying the expression of AQP3 may lead to an understanding of the role of the AQPs in human prostatic disease.

참고문헌 (Reference)

1 Verkman AS, "Structure and function of aquaporin water channels" 278 : 13-28, 2000

2 Schrier RW, "Renal aquaporin water channels: from molecules to human disease" 81 : 117-131, 2003

3 Cho YS, "Possible functional implications of aquaporin water channels in reproductive physiology and medically assisted procreation" 49 : 515-519, 2003

4 Jablonski EM, "Plasma membrane aquaporin activity can affect the rate of apoptosis but is inhibited after apoptotic volume decrease" 286 : 975-985, 2004

5 Nielsen S, "Physiology and pathophysiology of renal aquaporins" 10 : 647-663, 1999

6 Zardoya R, "Phylogeny and evolution of the major intrinsic protein family" 97 : 397-414, 2005

7 Verkman AS, "More than just water channels: unexpected cellular roles of aquaporins" 118 : 3225-3232, 2005

8 Ishibashi K, "Molecular cloning and expression of a member of the aquaporin family with permeability to glycerol and urea in addition to water expressed at the basolateral membrane of kidney collecting duct cells" 91 : 6269-6273, 1994

9 Ismail M, "Inhibition of the aquaporin 3 water channel increases the sensitivity of prostate cancer cells to cryotherapy" 100 : 1889-1895, 2009

10 Huang HF, "Function of aquaporins in female and male reproductive systems" 12 : 785-795, 2006

11 Wang J, "Expression of aquaporin 3 in the human prostate" 14 : 1088-1092, 2007

12 Jung SI, "Effects of castration on the expression of aquaporins and apoptosis in the ventral prostate" 9 : 238-244, 2012

13 Mobasheri A, "Distribution of AQP2 and AQP3 water channels in human tissue microarrays" 36 : 1-14, 2005

14 Jablonski EM, "Decreased aquaporin expression leads to increased resistance to apoptosis in hepatocellular carcinoma" 250 : 36-46, 2007

15 Verkman AS, "Aquaporinsnew players in cancer biology" 86 : 523-529, 2008

16 Agre P, "Aquaporin water channels: molecular mechanisms for human diseases" 555 : 72-78, 2003

17 Fulmer BR, "A blood-prostate barrier restricts cell and molecular movement across the rat ventral prostate epithelium" 163 : 1591-1594, 2000