I prepared a monoclonal antibody (mAb) A-l30 to HDL apolipoprotein A-l with the ultimate goals of expressing the valuable immunodiagnostic single-chain Fv (scFv) in Escherichia coli. The binding specificity of mAb A-l30 was determined by Western blot ...
I prepared a monoclonal antibody (mAb) A-l30 to HDL apolipoprotein A-l with the ultimate goals of expressing the valuable immunodiagnostic single-chain Fv (scFv) in Escherichia coli. The binding specificity of mAb A-l30 was determined by Western blot analysis. From the hybridoma cell line secreting mAb A-l30, poly(A)+ RNA was prepared and used as a template for cDNA synthesis and cloning. The nucleotide sequence analyses revealed that the variable regions of the heavy and light chains were members of mouse heavy chain subgroup ⅡA and κ light chain subgroup Ⅱ, respectively. Comparison of the nucleotide sequences with mouse immunoglobulin genes listed in the GenBank data base showed that the cDNAs have not been previously reported.