메틸렌블루 비색법을 이용한 원유중 음이온 계면활성제의 분석 = Determination of Anionic Surfactants in Raw Milks by Methylene Blue Colorimetry

다국어 초록 (Multilingual Abstract)

A determination method for anionic surfactants residue in raw milk was established using methylene blue method. The residue in raw milk was first precipitated with acetonitril, seperated by centrifugation, concentrated by evaporation of supernatant, dissolved with distilled water, allowed to complex formation by adding the methylene blue and extracted with chloroform. The final was analysed and quantified by spectrophotometry.
Based on the collaborative studies in 3 laboratories after spiking 5 to 20 ppm of standard solution, the mean of recovery rate within laboratory repeatability were 85.9%, 104.6% and 94.9%, respectively and the coefficient of variation were 9.3%, 9.8% and 6.4%. In addition, the coefficient of variation between laboratory repeatability was 16.1% which would be acceptable to the recommended guideline of CODEX.
In conclusion, the present method makes feasibilities for quantified and qualified determination of anionic surfactants residue in raw milk.