Screening and optimization of pretreatments in the preparation of sugarcane bagasse feedstock for biohydrogen production and process optimization

다국어 초록 (Multilingual Abstract)

<P><B>Abstract</B></P> <P>This work evaluated the effects of individual alkaline, sodium carbonate (Na<SUB>2</SUB>CO<SUB>3</SUB> denoted as; NaC), sodium sulfide (Na<SUB>2</SUB>SO<SUB>3</SUB> denoted as; NaS) and combination of NaC + NaS pretreatment for the saccharification of sugarcane bagasse (SCB). The effects of different pretreatments on chemical composition and structural complexity of SCB in relation with its saccharification were investigated. For enzymatic hydrolysis of pretreated SCB we have utilized the produced crude enzymes by <I>Streptomyces</I> sp. MDS to make the process more cost effective. A enzyme dose of 30 filter paperase (FPU) produced a maximum reducing sugar (RS) 592 mg/g with 80.2% hydrolysis yield from NaC + NaS pretreated SCB under optimized conditions. The resulted enzymatic hydrolysates of each pretreated SCB were applied for hydrogen production using <I>Clostridium beijerinckii</I> KCTC1785. NaC + NaS pretreated SCB hydrolysates exhibited maximum H<SUB>2</SUB> production relative to other pretreatment methods. Effects of temperature, initial pH of culture media and increasing NaC + NaS pretreated SCB enzymatic hydrolysates concentration (2.5–15 g/L) on bioH<SUB>2</SUB> production were investigated. Under the optimized conditions, the cumulative H<SUB>2</SUB> production, H<SUB>2</SUB> production rate, and H<SUB>2</SUB> yield were 1485 mL/L, 61.87 mL/L/h and 1.24 mmol H<SUB>2</SUB>/mol of RS (0.733 mmol H<SUB>2</SUB>/g of SCB), respectively. The efficient conversion of the SCB hydrolysate to H<SUB>2</SUB> without detoxification proves the viability of process for cost-effective hydrogen production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pretreatments of alkaline, sodium carbonate, sodium sulfide and combination demonstrated for sugarcane baggase. </LI> <LI> Several crucial factors towards optimization were carried out. </LI> <LI> Peak 59.2 g/L of reducing sugar (RS) with 80.2% hydrolysis yield from NaC + NaS pretreated SCB. </LI> <LI> Significant hydrogen production and process optimization using SCB hydrolysates studied in detail. </LI> </UL> </P>