This study was performed to develop culture medium which favors the growth of the Salmonella, Shigella and Vibrios, and is suitable for the detection of the carriers. The TCBS-S.S and the TCBS-MacConkey combined medium were trsted for the growth of th...
This study was performed to develop culture medium which favors the growth of the Salmonella, Shigella and Vibrios, and is suitable for the detection of the carriers. The TCBS-S.S and the TCBS-MacConkey combined medium were trsted for the growth of the bacteria and the ability to form colonies. The results of combined media were compared with those of each media as S-S, MacConkey and TCBS.
1. The growth of Salomonella typhi was excellent on TCBS-S.S and TCBS-MacConkey combined media, on the other hand it was markedly inhibited on S-S agar media.
2. The Shigella dysenteriae showed rapid growth and large colony formation on the combined media as TCBS-MacConkey and TCBS-S.S, but was inhibited on the other media.
3. Vibrio paraemolyticus grow well not only on the TCBS-MacConkey, TCBS-S.S combined media also on TCBS agar media.
4. Lactose-fermenting E. coli, sucrose-fermenting Proteus vulgaris and Vibro alginolyticus can be distinguishable by the color of colonies on TCBS-S.S combined agar media.
5. The colonies of Proteus mirabilis on TCBS-MacConkey combined agar media can be distinguishable from Proteus vulgaris by the dark green ring.
6. As Salmonella, Shigella and Vibrio grow well on TCBS-S.S combined media, they can be isolated on one plate of the media.