패턴인식법에 의한 시판 녹차의 산지 및 채엽시기 추정 = Estimation of Harvest Period and Cultivated Region of Commercial Green Tea by Pattern Recognition

다국어 초록 (Multilingual Abstract)

Quantitative analysis of (+)-catechin (C), (-)-epigallocatechin (EGC), (-)-epicatechin (EC), (-)-epigallocatechin gallate (EGCG), (-)-epicatechin gallate (ECG) and caffeine in commercial green tea was carried out by HPLC employing gradient elution of 0.1% acetic acid and acetonitrile on ODS column. The optimized HPLC method provided satisfactory linearity, accuracy and precision. The relationship between the concentration of the components and cultivated region of the commercial green tea was not significant, while the concentration of EGCG, ECG and caffeine decreased significantly in the later harvested green tea samples (p<0.01). Multivariate analysis of the components was performed in order to characterize and evaluate the cultivated region and harvest period-related variation. Hierarchical clustering and discriminant analysis were applied to classify the geographical and seasonal origins of the green tea samples. The classification accuracy of the cultivated region and harvest period by discriminant analysis was 95% and 91%, respectively, indicating that this method could be reliable and convenient for the quality control of herbal products with different origin.

참고문헌 (Reference)

1 권영민, "왕느릅나무 수피의 페놀성 화합물 및 항산화 활성" 한국생약학회 33 (33): 404-410, 2002

2 김상희, "보성산 녹차의 채엽시기에 따른 화학 성분의 변화" 한국식품과학회 36 (36): 542-546, 2004

3 Oh, M. J, "Variation of pectin, catechins and caffeine contents in Korean green tea (Camellia sinensis L.) by harvesting time and processing recipe" 40 : 775-, 1995

4 Oh, M. J, "Variation in chemical components of Korean green tea (Camellia sinensis L.) resulted from developing stages and processing recipe" 40 : 518-, 1995

5 Jochmann, N, "The efficacy of black tea in ameliorating endothelial function is equivalent to that of green tea" 99 : 863-, 2008

6 Shimizu, M, "Targeting receptor tyrosine kinases and multiple downstream signaling pathways by green tea catechin EGCG" 203-, 2007

7 Lee, Y. A, "Tannins from Rubus coreanum" 26 : 27-, 1995

8 Zhang, X., "Synergistic inhibition of head and neck tumor growth by green tea (-)- epigallocatechin-3-gallate and EGFR tyrosine kinase inhibitor" 123 : 1005-, 2008

9 Park, J. H, "Studies on chemical composition in Korean native tea plants" Chonnam National Univ. 1997

10 Chung, H. Y, "Studies on antioxidative and antimutagenic mechanism of epicatechin 3-O-gallate isolated from green tea" 65-, 1995

11 Talebpour, Z., "Simultaneous determination of theophylline and caffeine by proton magnetic resonance spectroscopy using partial least squares regression techniques" 19 : 1079-, 2003

12 Yamauchi, Y., "Simple and rapid UV spectrophotometry of caffeine in tea coupled with sample pre-treatment using a cartridge column filled with polyvinylpolypyrrolidone (PVPP)" 56 : 185-, 2008

13 Kim, J. T, "Science and culture of tea" Borim Press. 160-, 1996

14 "R version 2.6.1 (2007-11-26) Copyright (C) 2007 The R Foundation for Statistical Computing" 2007

15 Sultana, T, "Quality assessment and quantitative analysis of flavonoids from tea samples of different origins by HPLC -DAD-ESI-MS" 56 : 3444-, 2008

16 Chen, Q, "Qualitative identification of tea by near infrared spectroscopy based on soft independent modelling of class analogy pattern ecognition" 13 : 327-, 2005

17 Cho, H. S., "Protective effect of the green tea component, - theanine on environmental toxins-induced neuronal cell death" 29 : 656-, 2008

18 Ito, H., "Polyphenol levels in human urine after intake of six different polyphenol-rich beverages" 94 : 500-, 2005

19 Gao, D. F, "Phenolic antioxidants from green tea produced from Camellia taliensis" 56 : 7517-, 2008

20 Buchanan, R. L, "Methylxanthine inhibition of aflatoxin production" 43 : 654-, 1978

21 Munoz-Munoz, J. L., "Kinetic characterization of the enzymatic and chemical oxidation of the catechins in green tea" 56 : 9215-, 2008

22 Cho, Y. J., "Isolation and enzyme inhibition of tannins from Korean green tea" 26 : 216-, 1993

23 Chen, Q, "Identification of the green tea grade level using electronic tongue and pattern recognition" 41 : 500-, 2008

24 Dou, J, "Identification and comparison of phenolic compounds in the preparation of oolong tea manufactured by semifermentation and drying processes" 55 : 7462-, 2007

25 Pongsuwan, W, "High-throughput technique for comprehensive analysis of Japanese green tea quality assessment using ultra-performance liquid chromatography with time-of-flight mass spectrometry (UPLC/TOF MS)" 56 : 10705-, 2008

26 Ko, Y. S, "HPLC에 의한 증제와 볶음 녹차 중의 유리 아미노산과 유리당의 정량" 14 : 301-, 1985

27 우희섭, "HPLC에 의한 녹차의 polyphenol화합물의 분리 및 polyphenol의 생리활성" 한국식품과학회 35 (35): 1199-1203, 2003

28 Del Rio, D., "HPLC-MSn analysis of phenolic compounds and purine alkaloids in green and black tea" 52 : 2807-, 2004

29 Graham, H. N, "Green tea composition, consumption, and polyphenol chemistry" 21 : 334-, 1992

30 Adachi, N., "Green tea component, (-)- epigallocatechin gallate, but not L-theanine, has sedative effects in chick under acute stress conditions" 5 : 107-, 2007

31 Anderson, M. W., "Effect of dietary green tea extract and aerosolized difluoromethylornithine during lung tumor progression in A/J strain mice" 29 : 1594-, 2008

32 Asai, H., "Effect of alumina-tea catechin complex on the blood sugar in spontaneous diabetic mice" 21 : 163-, 1987

33 Lee, S. J, "Effect of Korean green tea, oolong tea and black tea beverage of the removal of cadmium and antioxidative detoxification in cadmium administered rats" 21-, 1995

34 Alcazar, A, "Differentiation of Green, White, Black, Oolong, and Pu-erh teas according to their free amino acids content" 55 : 5960-, 2007

35 Nakagawa, M, "Differences in amino acid, tannin, total nitrogen contents in leaves of cultivated species of green tea. Bull" 48 : 84-, 1975

36 Lee, B. L, "Comparative analysis of tea catechins and theaflavins by high-performance liquid chromatography and capillary electrophoresis" A 881 : 439-, 2000

37 Wee, J. H, "Catechin content and composition of domestic tea leaves at different packing time" 31 : 20-, 1999

38 Huang, Y., "Capillary electrophoretic separation of biologically active amines and acids using nanoparticle-coated capillaries" 29 : 1942-, 2008

39 Li, C, "Analysis of urinary metabolites of tea catechins by liquid chromatography/electrospray ionization mass spectrometry" 14 : 702-, 2001

40 Spacil, Z., "Analysis of phenolic compounds by HPLC and ultra performance liquid chromatography" 76 : 189-, 2008